Abstract
Despite their benefits, laborious preparation and purification procedures often make enzymes too expensive to be economically applicable. These efforts can be significantly reduced by immobilizing the enzymes on a microbial cell surface and employing the producing cells directly in the reaction. Here, we present recent progress made with autodisplay, an intensively used surface display technique for Gram-negative bacteria with some unique features that enable access to also difficult enzymes.
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Tozakidis, I.E.P., Quehl, P., Schüürmann, J. et al. Let’s do it outside: neue Biokatalysatoren mittels surface display . Biospektrum 21, 668–671 (2015). https://doi.org/10.1007/s12268-015-0628-1
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DOI: https://doi.org/10.1007/s12268-015-0628-1