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Angestaubt, aber nicht eingerostet — der Bioreporter LacZ

  • Wissenschaft · Methoden
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Abstract

β-galactosidase encoded by lacZ remains a popular reporter enzyme. Here, we present a convenient tool box to facilitate rapid construction of reporter lacZ fusions. The first tool enables the simple generation of chromosomally encoded reporters within the Escherichia coli lac operon using Red®/ET® recombination. The second tool allows the introduction of lacZ reporter fusions into the chromosome by a single-crossover method. The third tool comprises four pBBR1-based broad-host-range vectors to derive transcriptional and translational lacZ fusions.

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Authors and Affiliations

  1. Munich Center for Integrated Protein Science (CIPSM) und Department für Biologie I, Mikrobiologie, Ludwig-Maximilians-Universität München, Großhaderner Straße 2-4, D-82152, Martinsried, Deutschland

    Jürgen Lassak, Luitpold Fried & Kirsten Jung

  2. Dust Biosolutions GmbH, Martinsried, Deutschland

    Luitpold Fried

Authors
  1. Jürgen Lassak
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  2. Luitpold Fried
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  3. Kirsten Jung
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Corresponding author

Correspondence to Kirsten Jung.

Additional information

Jürgen Lassak Biologiestudium an der Universität Tübingen. 2007–2010 Promotion am Max-Planck-Institut für Terrestrische Mikrobiologie in Marburg. Seit 2010 wissenschaftlicher Mit arbeiter in der Gruppe von Prof. Dr. K. Jung an der LMU München als Postdoktorand und seit 2013 als Akademischer Rat.

Luitpold Fried Biologiestudium an der LMU München, dort 2008–2013 Doktorand und Postdoktorand in der Gruppe von Prof. Dr. K. Jung. Seit 2013 technischer Leiter der Dust Bio-Solutions GmbH in München.

Kirsten Jung Biochemiestudium und Promotion an der Universität Leipzig. 1992–1994 Postdoktorandenaufenthalt am Howard Hughes Medical Institute, University of California, Los Angeles, USA. 1994–2002 Wissenschaftliche Mitarbeiterin und Heisenbergstipendiatin an der Universität Osnabrück. 2002–2004 Professorin für Mikrobiologie an der TU Darmstadt. Seit 2004 Lehrstuhl inhaberin für Mikrobiologie an der LMU München.

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Lassak, J., Fried, L. & Jung, K. Angestaubt, aber nicht eingerostet — der Bioreporter LacZ. Biospektrum 20, 510–513 (2014). https://doi.org/10.1007/s12268-014-0473-7

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  • DOI: https://doi.org/10.1007/s12268-014-0473-7

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