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Einzelzellen für die forensische DNA-Analyse direkt vom Spurenträger

  • Wissenschaft · Special: Forensik
  • Rechtsmedizin
  • Published:
BIOspektrum Aims and scope

Zusammenfassung

Mithilfe eines Mikroskops mit einem digitalen Mikromanipulator werden einzelne Epithelzellen von Spurenträgern gesichert. Die forensische DNA-Analyse der Einzelzellen erfolgt mittels Sequenzierung der hypervariablen Region der mtDNA.

Abstract

With a technique using a microscope with a digital micromanipulator, single epithelial cells can be lifted off the surface of material evidence and can be directly made available for forensic DNA-testing. DNA-testing is then accomplished by sequencing the hypervariable region of the mtDNA.

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Literatur

  1. Brinkmann B (2004) Forensische DNA-Analytik. Dtsch Arztebl 101:A2329–A2335

    Google Scholar 

  2. Oberacher H, Niederstäter H, Parson W (2007) Liquid chromatography-electrospray ionization mass spectrometry for simultaneous detection of mtDNA length and nucleotide polymorphisms. Int J Legal Med 121:57–67

    Article  PubMed  Google Scholar 

  3. Parson W, Brandstätter A, Alonso A et al. (2004) The EDNAP mitochondrial DNA population database (EMPOP) collaborative exercises: organisation, results and perspectives. Forensic Sci Int 139:215–226

    Article  PubMed  CAS  Google Scholar 

  4. Szibor R, Plate I, Heinrich M et al. (2007) Mitochondrial D-Loop (CA)n repeat length heteroplasmy: frequency in a German population sample and inheritance studies in two pedigrees. Int J Legal Med 121:207–213

    Article  PubMed  Google Scholar 

  5. Szibor R, Plate I, Schmitter H et al. (2006) Forensic mass screening using mtDNA. Int J Legal Med 120:372–376

    Article  PubMed  Google Scholar 

  6. Brück S, Evers H, Heidorn F et al. (2011) Single cells for forensic DNA analysis — from evidence material to test tube. J Forensic Sci 56:176–180

    Article  PubMed  Google Scholar 

  7. Andréasson H, Nilsson M, Budowle B et al. (2006) Quantification of mtDNA mixtures in forensic evidence material using pyrosequencing. Int J Legal Med 120:183–390

    Article  Google Scholar 

  8. Brück S, Thias V, Heidorn F et al. (2010) Sequenzierung der HV1- und HV2-Region der mtDNA an einzelnen Epithelzellen. Rechtsmedizin 20:25–33

    Article  Google Scholar 

  9. Lutz-Bonengel S, Sänger T, Parson W et al. (2008) Single lymphocytes from two healthy individuals with mitochondrial point heteroplasmy are mainly homoplasmatic. Int J Legal Med 122:189–197

    Article  PubMed  Google Scholar 

  10. Lutz-Bonengel S, Sänger T, Heinrich M et al. (2007) Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip. Int J Legal Med 121:68–73

    Article  PubMed  Google Scholar 

  11. Schmidt U, Lutz-Bonengel S, Weisser HJ et al. (2006) Low-volume amplification on chemically structured chips using the PowerPlex 16 DNA amplification kit. Int J Legal Med 120:42–48

    Article  PubMed  Google Scholar 

  12. Becker I, Becker KF, Röhrl MH et al. (1997) Laserassisted preparation of single cells from stained histological slides for gene analysis. Histochem Cell Biol 108:447–451

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Marcel A. Verhoff.

Additional information

Marcel A. Verhoff Jahrgang 1970. Medizinstudium an der Universität Gießen. 1999 Promotion in der Physiologie. Seit 2000 wissenschaftlicher Mitarbeiter im Institut für Rechtsmedizin der Universität Gießen. 2003 Facharzt für Rechtsmedizin. 2007 Habilitation im Fach Rechtsmedizin. 2008 apl. Professor.

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Dambor, T., Brück, S., Heidorn, F. et al. Einzelzellen für die forensische DNA-Analyse direkt vom Spurenträger. Biospektrum 17, 185–187 (2011). https://doi.org/10.1007/s12268-011-0029-z

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  • DOI: https://doi.org/10.1007/s12268-011-0029-z

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