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Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

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Abstract

Low volume (LV) amplification (1 μL) of nuclear DNA (nucDNA) on a chemically structured chip is an appropriate and highly sensitive method to simultaneously amplify amelogenin and 15 forensically relevant short tandem repeats (STR). In this study, a combined method using on-chip LV amplification of mitochondrial DNA (mtDNA) and subsequent on-chip LV cycle sequencing was established to obtain a method, which is sensitive and robust enough to allow reliable analysis of DNA amounts representing the single cell level. All the necessary steps of the procedure—except for the purification of the sequencing products—were accomplished within the same final 2-μL reaction volume.

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Authors and Affiliations

  1. Institute of Legal Medicine, Albert Ludwig University Freiburg, Albertstrasse 9, 79104, Freiburg, Germany

    Sabine Lutz-Bonengel, Timo Sänger, Marielle Heinrich & Ulrike Schmidt

  2. Alopex GmbH, Fritz-Hornschuch-Strasse 9, 95326, Kulmbach, Germany

    Ulrike Schön

Authors
  1. Sabine Lutz-Bonengel
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  2. Timo Sänger
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  3. Marielle Heinrich
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  4. Ulrike Schön
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  5. Ulrike Schmidt
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Correspondence to Sabine Lutz-Bonengel.

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Lutz-Bonengel, S., Sänger, T., Heinrich, M. et al. Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip. Int J Legal Med 121, 68–73 (2007). https://doi.org/10.1007/s00414-006-0125-7

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  • DOI: https://doi.org/10.1007/s00414-006-0125-7

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