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Correlation Between Wall Shear Stress and Acute Degradation of the Endothelial Glycocalyx During Cardiopulmonary Bypass

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Abstract

Wall shear stress (WSS) plays a key role in maintaining glycocalyx function, gene expression, and structure. Experimental studies have discussed the relationship between the shedding of the endothelial glycocalyx (EG) and WSS. However, rare literature about how WSS affects the EG during cardiopulmonary bypass (CPB) was mentioned. This study aimed to investigate the correlation between the WSS of carotid arteries and shedding of the EG during CPB in humans. The WSS level was calculated in accordance with an equation. The plasma concentrations of heparan sulfate, syndecan-1, and nitric oxide were measured to reflect shedding of the EG at six time points. A negative correlation was observed between the peak wall shear stress (PWSS) and syndecan-1 (R = − 0.5, p < 0.01) and heparan sulfate (R = − 0.461, p < 0.01) during CPB. The WSS is closely associated with the components of glycocalyx shedding during CPB. The WSS produced by non-pulsatile flow during CPB may contribute to the degradation of EG.

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Abbreviations

WSS:

Wall shear stress

PWSS:

Peak wall shear stress

MWSS:

Mean wall shear stress

EG:

Endothelial glycocalyx

CPB:

Cardiopulmonary bypass

HS:

Heparan sulfate

NO:

Nitric oxide

ECG:

Electrocardiogram

ICU:

Intensive care unit

SIRS:

Systemic inflammatory reaction syndrome

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Acknowledgment

We wish to thank the Medical Research Collaborating Center, The First Affiliated Hospital of Wenzhou Medical University, for providing statistical consultation services.

Funding

This study was supported by the Wenzhou Science and Technology Bureau of China (No.y20160134).

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Authors and Affiliations

  1. Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China

    Guoliang He, Yuan Gao, Linya Feng, Guodong He, Qiaolin Wu, Wei Gao, Lina Lin & Weijian Wang

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  1. Guoliang He
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  2. Yuan Gao
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  3. Linya Feng
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  4. Guodong He
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  5. Qiaolin Wu
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  6. Wei Gao
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  7. Lina Lin
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  8. Weijian Wang
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Contributions

(I) Conception and design: GL He and WJ Wang

(II) Administrative support: WJ Wang

(III) Provision of study materials or patients: GL He, LN Lin, and WJ Wang;

(IV) Collection and assembly of data: GL He and LN Lin

(V) Data analysis and interpretation: GL He and WJ Wang

(VI) Manuscript writing: All authors

(VII) Final approval of manuscript: All authors

Corresponding authors

Correspondence to Lina Lin or Weijian Wang.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

The study protocol was reviewed by our institutional review board and approved as a prospective study (approval number: 2016056). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Associate Editor Junjie Xiao oversaw the review of this article

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The original version of this article was revised: This article was updated after its original publication to add Lina Lin as co-corresponding author (at: wzlinlina@163.com).

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He, G., Gao, Y., Feng, L. et al. Correlation Between Wall Shear Stress and Acute Degradation of the Endothelial Glycocalyx During Cardiopulmonary Bypass. J. of Cardiovasc. Trans. Res. 13, 1024–1032 (2020). https://doi.org/10.1007/s12265-020-10027-2

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