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Involvement of Histamine 2 Receptor in Alpha 1 Adrenoceptor Mediated Cardiac Hypertrophy and Oxidative Stress in H9c2 Cardio Myoblasts

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Abstract

Despite the involvement of ɑ1adrenergic (ɑ1AR) and Histamine 2 receptors (H2R) in cardiac hypertrophy (CH), their relationship is yet to be studied. Our study investigated interrelationship between them using in vitro CH model. H9c2 cardiomyoblasts were exposed to phenylephrine (ɑ1AR agonist-50 μM) in the presence, the absence of famotidine (H2R antagonist-10 μM) and BAY 11-7082 (NF-kB inhibitor-10 μM). The impact of ɑ1AR stimulation on H2R expression and oxidative stress was assessed. Hypertrophic indices were assessed from activities of enzymatic mediators of cardiac hypertrophy, total protein content, BNP levels and cell volume. Additionally, the inverse agonistic property of famotidine and NFkB activity was also studied. ɑ1AR-induced H2R expression, oxidative stress and hypertrophic indices were significantly abolished by famotidine and pharmacological inhibitor of NFkB. Increase in constitutive activity of H2R was noticed correlating with increased receptor population. These results suggest involvement of NFkB-mediated upregulation of H2R in ɑ1AR-mediated CH.

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Abbreviations

AR:

Adrenergic receptors

BST:

Bryostatin 1

BUR:

Burimamide

Fam:

Famotidine

H2R:

Histamine 2 receptor

PE:

Phenylephrine

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Acknowledgements

The authors would like to acknowledge and show gratitude for the support extended by the National Institute of Pharmaceutical Education and Research, Hyderabad for permitting us to utilize their research facilities.

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Authors and Affiliations

  1. Department of Animal Physiology, Indian Council for Medical Research—National Animal Resource Facility for Biomedical Research, National Institute of Nutrition Campus, Turkapally Village, Shameerpet, Hyderabad, Telangana, India

    Ajay Godwin Potnuri

  2. Department of Pharmacology, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana, India

    Lingesh Allakonda

  3. Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, 35294, USA

    Sherin Saheera

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  1. Ajay Godwin Potnuri
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Correspondence to Ajay Godwin Potnuri.

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ESM 1

Raw Western Blot images. (a-b) the pictorial representation of PRX3 and corresponding Beta actin (c-d)) the pictorial representation of H2R and corresponding Beta actin (PNG 1790 kb)

High Resolution (TIF 1653 kb)

ESM 2

Melt curve images. (a) the pictorial representation of melt curves for fig. 7c (b)) the pictorial representation of melt curves for fig. 7d (JPG 220 kb)

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Potnuri, A.G., Allakonda, L. & Saheera, S. Involvement of Histamine 2 Receptor in Alpha 1 Adrenoceptor Mediated Cardiac Hypertrophy and Oxidative Stress in H9c2 Cardio Myoblasts. J. of Cardiovasc. Trans. Res. 14, 184–194 (2021). https://doi.org/10.1007/s12265-020-09967-6

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