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Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification

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Abstract

Bacillus pumilus, a bacterial strain was isolated from agricultural soil and used for xylanase enzyme (Xy) production under the submerged fermentation technique. The (Xy) enzyme had an optimum temperature at 50℃ (maximum activity from 45–60 °C) and was active at broad pH range (5.0–8.0) with an optimum pH at around 6.3 as evaluated from response surface methodology studies. This enzyme after purification (purification; 2.87 folds, specific activity; 64.3 U/mg) was immobilized onto MOFCu-BTC (a copper ion-based metal organic framework) and was used for clarification of freshly squeezed fruit juice (pineapple and pomegranate). The study revealed an improved catalytic efficiency (Vmax from 1.252.5 to 1.361 U/mL/mg of support) and greater half-life of the immobilized system (77–99 min). The activation energy decreased from that required for the free system (37.59–25.63 kJ/mol). The reusability of the enzyme improved after immobilization where 61% of the enzyme’s activity was retained after 21 cycles of usage. The MOFXy-Cu-BTC system showed improved clarification (47.58–57.97% for pineapple, and 15.34–18.3 for pomegranate) thereby showing its effectiveness in commercial juice clarification process.

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Acknowledgements

This work is supported by WOS-A DST fellowship LS-2/2021 and DBT Builder Project (BT/INF/ 22/SP41295/2020) at University Institute of Engineering and Technology, Panjab University, India.

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Authors and Affiliations

  1. Department of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, 160014, India

    Jyoti Kaushal, Madhu Khatri & Shailendra Kumar Arya

  2. Department of Medical Laboratory Sciences, Lovely Professional University, Phagwara, 144411, Punjab, India

    Gursharan Singh

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  1. Jyoti Kaushal
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  2. Madhu Khatri
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  4. Shailendra Kumar Arya
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SKA, MK and GS designed the concept; JK wrote the manuscript. All of the authors discussed, commented on, and revised the manuscript.

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Correspondence to Shailendra Kumar Arya.

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Kaushal, J., Khatri, M., Singh, G. et al. Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification. Biotechnol Bioproc E 29, 197–210 (2024). https://doi.org/10.1007/s12257-024-00007-7

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