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Production of Lipid Containing High Levels of Docosahexaenoic Acid by Cultivation of Aurantiochytrium sp. KRS101 Using Jerusalem Artichoke Extract

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Abstract

In the present study, we evaluated extract of Jerusalem artichoke tubers (JAT) as a substrate for the production of lipid containing high levels of docosahexaenoic acid (DHA) by cultivated Aurantiochytrium sp. KRS101, an oleaginous protist. The optimal conditions for cultivation determined using response surface methods were as follows: pH, 5.9; enzyme loading, 282.6; and temperature, 27.7°C. The maximal levels of lipid (16.4 g/L) and productivity (3.6 g/L/d) were obtained during fed-batch fermentation, which achieved a DHA yield of 7.9 g/L (>48% of total fatty acids by weight). Moreover, JAT was more economical, reducing costs of expensive yeast extract as a nitrogen source by about 40%. These results suggest that JAT may be further explored as a low-cost feedstock for lipid production using microalgae strains.

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Author notes

  1. These authors are co-first authors and contributed equally.

Authors and Affiliations

  1. Industrial Microbiology & Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580-185, Korea

    Jung-Hyun Ju, Baek-Rock Oh, Seung-Kyu Ryu, Sun-Yeon Heo, Su-Yeon Kim, Chul Ho Kim & Jeong-Woo Seo

  2. Biosystems and Bioengineering KRIBB School, University of Science and Technology (UST), Daejeon, 34113, Korea

    Su-Yeon Kim

  3. Korea Zoonosis Research Institute (KoZRI), Chonbuk National University, Iksan, 54531, Korea

    Won-Kyung Hong

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  1. Jung-Hyun Ju
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  2. Baek-Rock Oh
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  3. Seung-Kyu Ryu
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  4. Sun-Yeon Heo
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Correspondence to Chul Ho Kim or Jeong-Woo Seo.

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Ju, JH., Oh, BR., Ryu, SK. et al. Production of Lipid Containing High Levels of Docosahexaenoic Acid by Cultivation of Aurantiochytrium sp. KRS101 Using Jerusalem Artichoke Extract. Biotechnol Bioproc E 23, 726–732 (2018). https://doi.org/10.1007/s12257-018-0419-x

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  • DOI: https://doi.org/10.1007/s12257-018-0419-x

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