Skip to main content
Log in

Production of lipids containing high levels of docosahexaenoic acid from empty palm fruit bunches by Aurantiochytrium sp. KRS101

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

The oleaginous microalga Aurantiochytrium sp. KRS101 was cultivated in enzymatic hydrolysates of alkali-pretreated empty palm fruit bunches (EFBs), without prior detoxification process. The maximal levels of lipid and docosahexaenoic acid synthesized were 12.5 and 5.4 g L−1 after cultivation for 36 h. Similar lipid levels were also obtained via simultaneous saccharification and cultivation. The results suggested that EFB is a promising source for production of useful lipids by the microalgal strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Gouda MK, Omar SH, Aouad LM (2008) Single cell oil production by Gordonia sp. DG using agro-industrial wastes. World J Microbiol Biotechnol 24:1703–1711

    Article  CAS  Google Scholar 

  2. Xu H, Miao X, Wu Q (2006) High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J Biotechnol 126:499–507

    Article  CAS  Google Scholar 

  3. Yokochi T, Honda D, Higashihara T, Nakahara T (1998) Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21. Appl Microbiol Biotechnol 49:72–76

    Article  CAS  Google Scholar 

  4. Brooks SL, Mitchell A, Steffenson N (2000) Mothers, infants, and DHA. Implications for nursing practice. Am J Matern Child Nurs 25:71–75

    Article  CAS  Google Scholar 

  5. Carlson SE (2009) Early determinants of development: a lipid perspective. Am J Clin Nutr 89:678S–684S

    Article  CAS  Google Scholar 

  6. Auestad N, Halter R, Hall RT, Blatter M, Bogle ML, Burks W, Erickson JR, Fitzgerald KM, Dobson V, Innis SM, Singer LT, Montalto MB, Jacobs JR, Qiu W, Bornstein MH (2001) Growth and development in term infants fed long-chain polyunsaturated fatty acids: a double-masked, randomized, parallel, prospective, multivariate study. Pediatrics 108:372–381

    Article  CAS  Google Scholar 

  7. Zhao ZB, Hua YY, Liu B (2005) Strategies to secure feedstock supply for Chinese biodiesel industry. China Biotechnol 25:1–6

    CAS  Google Scholar 

  8. Hong WK, Rairakhwada D, Seo PS, Park SY, Hur BK, Kim CH, Seo JW (2011) Production of lipids containing high levels of docosahexaenoic acid by a newly isolated microalga, Auratiochytrium sp. KRS101. Appl Biochem Biotechnol 164:1468–1480

    Article  CAS  Google Scholar 

  9. Liang Y, Sarkany N, Cui Y, Yesuf J, Trushenski J, Blackburn J (2010) Use of sweet sorghum juice for lipid production by Schizochytrium limacinum SR21. Bioresour Technol 101:3623–3627

    Article  CAS  Google Scholar 

  10. Hong WK, Kim CH, Yu AN, Sohn JH, Yang JW, Kondo A, Seo JW (2012) Production of lipid with high level of docosahexaenoic acid by large-scale cultivation of Aurantiochytrium sp. KRS101 (Submitted)

  11. Chi Z, Hu B, Liu Y, Frear C, Wen Z, Chen S (2007) Production of omega-3 polyunsaturated fatty acids from cull potato using an algae culture process. Appl Biochem Biotechnol 137–140:805–815

    Article  Google Scholar 

  12. Huang C, Chen XF, Xiong L, Chen XD, Ma LL (2012) Oil production by the yeast Trichosporon dermatis cultured in enzymatic hydrolysates of corncobs. Bioresour Technol 110:711–714

    Article  CAS  Google Scholar 

  13. Huang C, Zong MH, Wu H, Liu QP (2009) Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour Technol 100:4535–4538

    Article  CAS  Google Scholar 

  14. Anastasi A, Spina F, Romagnolo A, Tigini V, Prigione V, Varese GC (2012) Integrated fungal biomass and activated sludge treatment for textile wastewaters bioremediation. Bioresour Technol 123:106–111

    Article  CAS  Google Scholar 

  15. Burja AM, Radianingtyas H, Windust A, Barrow CJ (2006) Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species: screening of strains and optimization of omega-3 production. App Microbiol Biotechnol 72:1161–1169

    Article  CAS  Google Scholar 

  16. Die C, Tao J, Xie F, Dai Y, Zhao M (2007) Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. Afr J Biotechnol 6:2130–2134

    Google Scholar 

  17. Li P, Miao X, Li R, Zhong J (2011) In Situ biodiesel production from fast-growing and high oil content Chlorella pyrenoidosa in rice straw hydrolysate. J Biomed Biotechnol 2011:1–8. doi:10.1155/2011/141207

  18. Tsigie YA, Wang CY, Truong CT, Ju YH (2011) Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate. Bioresour Technol 102:9216–9222

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported the Advanced Biomass R&D Center of Korea (Grant No. 2010-0029737) funded by the Ministry of Education, Science and Technology and by the Ministry of Food, Agriculture, Forestry, and Fisheries, of the Republic Korea.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jeong-Woo Seo.

Additional information

W.-K. Hong and A. Yu contributed equally to this article.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hong, WK., Yu, A., Heo, SY. et al. Production of lipids containing high levels of docosahexaenoic acid from empty palm fruit bunches by Aurantiochytrium sp. KRS101. Bioprocess Biosyst Eng 36, 959–963 (2013). https://doi.org/10.1007/s00449-012-0830-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-012-0830-1

Keywords

Navigation