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Enhanced Production of β-D-glycosidase and α-L-arabinofuranosidase in Recombinant Escherichia coli in Fed-batch Culture for the Biotransformation of Ginseng Leaf Extract to Ginsenoside Compound K

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Abstract

Ginsenoside compound K is an essential ingredient in nutritional supplements, cosmetics, and traditional medicines. However, cultivation for the production of enzymes involved in ginsenoside biotransformation has not been attempted in a fermenter. The host strain Escherichia coli ER2566 and the constitutive pHCE vector were selected for the efficient production of β-D-glycosidase, and expression medium composition to produce Sulfolobus solfataricus β-glycosidase expressed in E. coli was optimized in flask and batch cultures. The total activity of β-Dglycosidase in fed-batch culture using a fermenter increased 14-fold before optimization. S. solfataricus β-D-glycosidase and Thermotoga petrophila α-L-arabinofuranosidase were produced in a fed-batch culture. These two enzymes completely converted protopanaxadiol-type ginsenosides in ginseng leaf extract obtained from discarded ginseng leaves as a renewable substrate to compound K. The effective bioprocess for compound K production developed here will contribute to the industrial biological production of compound K.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Korea

    Tae-Hun Kim, Eun-Joo Yang, Kyung-Chul Shin & Deok-Kun Oh

  2. Skin Research Institute, Amorepacific Corporation R&D Center, Yongin, 17074, Korea

    Kyeong-Hwan Hwang & Jun Seong Park

Authors
  1. Tae-Hun Kim
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  2. Eun-Joo Yang
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  3. Kyung-Chul Shin
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  4. Kyeong-Hwan Hwang
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  5. Jun Seong Park
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  6. Deok-Kun Oh
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Correspondence to Deok-Kun Oh.

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Electronic Supplementary Material (ESM) The online version of this article (doi: 10.1007/s12257-018-0027-9) contains supplementary material, which is available to authorized users.

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Kim, TH., Yang, EJ., Shin, KC. et al. Enhanced Production of β-D-glycosidase and α-L-arabinofuranosidase in Recombinant Escherichia coli in Fed-batch Culture for the Biotransformation of Ginseng Leaf Extract to Ginsenoside Compound K. Biotechnol Bioproc E 23, 183–193 (2018). https://doi.org/10.1007/s12257-018-0027-9

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  • DOI: https://doi.org/10.1007/s12257-018-0027-9

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