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Heterologous expression and characterization of a recombinant thermophilic arylsulfatase from Thermotoga maritima

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Abstract

Production of low sulfated agar or agarose from agar or agaropectins by enzymatic hydrolysis has advantages but a high melting temperature is needed. The arylsulfatase gene from thermophilic Thermotoga maritima was cloned and expressed in Escherichia coli W3110 with pCol-MICT as the vector. The gene was comprised of 1,782 bp and encoded a protein of 593 amino acids with a molecular weight of 65 kDa. The recombinant arylsulfatase was partially purified by heat treatment (70°C, 30 min) and characterized. The enzyme was prepared with a total protein content of 2.4 mg and a specific activity of 20.63 U/mg. Optimal temperature and pH of the enzyme were 80°C and 7.0, respectively, for hydrolysis of p-nitrophenyl sulfate and sulfate content of agar was diminished to 40% after a 12 h treatment at that condition. Enhanced electrophoretic movement of DNA was observed in enzymetreated agar gel compared to that in a non-treated agar gel. These results suggest that thermophilic arylsulfatase expressed in E. coli could be useful for producing a low sulfated agar and electrophoretic grade agarose.

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Authors and Affiliations

  1. Deaprtment of Pharmaceutical Engineering, College of Medical Life Sciences, Silla University, Busan, 617-736, Korea

    Dong-Geun Lee, Jae Gyun Shin & Sang-Hyeon Lee

  2. Major in Bioscience and Biotechnology, Graduate School, Silla University, Busan, 617-736, Korea

    Dong-Geun Lee, Myong Je Jeon & Sang-Hyeon Lee

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  1. Dong-Geun Lee
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  2. Jae Gyun Shin
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Correspondence to Sang-Hyeon Lee.

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Lee, DG., Shin, J.G., Jeon, M.J. et al. Heterologous expression and characterization of a recombinant thermophilic arylsulfatase from Thermotoga maritima . Biotechnol Bioproc E 18, 897–902 (2013). https://doi.org/10.1007/s12257-013-0094-x

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  • DOI: https://doi.org/10.1007/s12257-013-0094-x

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