Abstract
The gene for β-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than that in E. coli. The enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose from agar. Neoagarooligosaccharides produced by the enzyme had a whitening effect and inhibited tyrosinase activity in the murine melanoma cell line, B16F10. Neoagarooligosaccharides were not cytotoxic to B16F10 or normal cells. β-Agarase could therefore be a good whitening, cosmetic additive.
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This work was supported by the Marine and Extreme Genome Research Center Program, Ministry of Maritime Affairs & Fisheries, Republic of Korea.
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Lee, DG., Jang, M.K., Lee, OH. et al. Over-production of a glycoside hydrolase family 50 β-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product. Biotechnol Lett 30, 911–918 (2008). https://doi.org/10.1007/s10529-008-9634-4
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DOI: https://doi.org/10.1007/s10529-008-9634-4