Abstract
A bacterial culture of Nitrosomonas sp. was isolated from a nitrifying biofilm to construct a biosensor for ammonium nitrogen (NH +4 −N) measurements in high ammonia wastewaters. The pure culture of microorganisms was immobilized into agarose gel matrix to attain a stable biosensor with a long service life. Biosensors were calibrated using (NH4)2SO4 solution and a steady-state method. Subsequently, several experiments with synthetic and industrial wastewaters were conducted. A linear range up to 20 mg/L of NH +4 −N, and sensitivities between 0.030 and 0.036 were gained with biosensors. During 14 days of stable service life of the Nitrosomonas sp. biosensors, variation of the signal was less than 7%. Response times of biosensors were 15 ∼ 25 min, while recovery times were up to 25 min. Measurements with high ammonia content synthetic and industrial wastewaters were conducted, and 8.3 and 5.6% over estimation of NH +4 −N was gained, respectively, compared with results of Nessler method. In spite of the small overestimation, the biosensor based on a pure culture of Nitrosomonas sp. and calibrated with (NH4)2SO4 is suitable for the analysis of NH +4 −N in high ammonia content wastewaters.
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Lin, L., S. Yuan, J. Chen, Z. Xu, and X. Lu (2009) Removal of ammonia nitrogen in wastewater by microwave radiation. J. Hazard. Mater. 161: 1063–1068.
Schmidt, I., O. Sliekers, M. Schmid, E. Bock, J. Fuerst, J. G. Kuenen, M. S. M. Jetten, and M. Strous (2003) New concepts of microbial treatment processes for the nitrogen removal in wastewater. FEMS Microbiol. Rev. 27: 481–492.
Xinshan, S., L. Qin, and Y. Denghua (2010) Nutrient removal by hybrid subsurface flow constructed wetlands for high concentration ammonia nitrogen wastewater. Procedia Environ. Sci. 2: 1461–1468.
Timmer, B., W. Olthuis, and A. V. D. Berg (2005) Ammonia sensors and their applications—a review. Sens. Actuators B. 107: 666–677.
Thévenot, D. R., K. Toth, R. A. Durst, and G. S. Wilson (2001) Electrochemical biosensors: Recommended definitions and classification. Biosens. Bioelectron. 16: 121–131.
D’Souza, S. F. (2001) Microbial biosensors. Biosens. Bioelectron. 16: 337–353.
Raud, M., E. Linde, E. Kibena, S. Velling, T. Tenno, E. Talpsep, and T. Kikas (2010) Semi-specific biosensors for measuring BOD in dairy wastewater. J. Chem. Technol. Biotechnol. 85: 957–961.
Raud, M., T. Tenno, E. Jõgi, and T. Kikas (2012) Comparative study of semi-specific Aeromonas hydrophila and universal Pseudomonas fluorescens biosensors for BOD measurements in meat industry wastewaters. Enz. Microbial. Technol. 50: 221–226.
Liu, J. and B. Mattiasson (2002) Microbial BOD sensors for wastewater analysis. Water Res. 36: 3786–3802.
Raud, M., M. Tutt, E. Jõgi, and T. Kikas (2012) BOD biosensors for pulp and paper industry wastewater analysis. Environ. Sci. Poll. Res. 19: 3039–3045.
Dubey, R. S. and S. N. Upadhyay (2001) Microbial corrosion monitoring by an amperometric microbial biosensor developed using whole cell of Pseudomonas sp. Biosens. Bioelectron. 16: 995–1000.
Orupõld, K., A. Mashirin, and T. Tenno (1995) Amperometric phenol sensor with immobilized bacteria. Electroanal. 7: 904–906.
Yüce, M., H. Nazır, and G. Dönmez (2010) A voltammetric Rhodotorula mucilaginosa modified microbial biosensor for Cu(II) determination. Bioelectrochem. 79: 66–70.
Eltzov, E., R. S. Marks, S. Voost, B. A. Wullings, and M. B. Heringa (2009) Flow-through real time bacterial biosensor for toxic compounds in water. Sens. Actuators B. 142: 11–18.
Bollmann, A. and N. P. Revsbech (2005) An NH4+ biosensor based on ammonia-oxidizing bacteria for use under anoxic conditions. Sens. Actuators B. 105: 412–418.
Dong, N. M., N. Risgaard-Petersen, J. Sørensen, and K. K. Brandt (2010) Rapid and sensitive nitrosomonas europaea biosensor assay for quantification of bioavailable ammonium sensu strictu in soil. Environ. Sci. Technol. 45: 1048–1054.
Grunditz, C. and G. Dalhammar (2001) Development of nitrification inhibition assays using pure cultures of nitrosomonas and nitrobacter. Water Res. 35: 433–440.
Cui, R., W. -J. Chung, and D. Jahng (2005) A rapid and simple respirometric biosensor with immobilized cells of Nitrosomonas europaea for detecting inhibitors of ammonia oxidation. Biosens. Bioelectron. 20: 1788–1795.
Su, L., W. Jia, C. Hou, and Y. Lei (2011) Microbial biosensors: A review. Biosens. Bioelectron. 26: 1788–1799.
Hsieh, Y. L., S. K. Tseng, and Y. J. Chang (2002) Nitrification using polyvinyl alcohol-immobilized nitrifying biofilm on an O_2-enriching membrane. Biotechnol. Lett. 24: 315–319.
Lagarde, F. and N. Jaffrezic-Renault (2011) Cell-based electrochemical biosensors for water quality assessment. Anal. Bioanal. Chem. 400: 947–964.
Tokuyama, T., A. Mine, K. Kamiyama, R. Yabe, K. Satoh, H. Matsumoto, R. Takahashi, and K. Itonaga (2004) Nitrosomonas communis strain YNSRA, an ammonia-oxidizing bacterium, isolated from the reed rhizoplane in an aquaponics plant. J. Biosci. Bioeng. 98: 309–312.
Tomiyama, H., M. Ohshima, S. Ishii, K. Satoh, R. Takahashi, K. Isobe, H. Iwano, and T. Tokuyama (2001) Characteristics of newly isolated nitrifying bacteria from rhizoplane of paddy rice. Microb. Environ. 16: 101–108.
Satoh, K., R. Takizawa, M. Sarai, N. Sato, R. Takahashi, and T. Tokuyama (2004) Two kinds of ammonia-oxidizing bacteria isolated from biologically deodorizing plants in cold district. J. Biosci. Bioeng. 98: 207–210.
Kibena, E., M. Raud, E. Jõgi, and T. Kikas (2013) Semi-specific M. phyllosphaerae based microbial sensor for biochemical oxygen demand measurements in dairy wastewater. Environ. Sci. Poll. Res. 20: 2492–2498.
Chan, C., M. Lehmann, K. Chan, P. Chan, C. Chan, B. Gruendig, G. Kunze, and R. Renneberg (2000) Designing an amperometric thick-film microbial BOD sensor. Biosens. Bioelectron. 15: 343–353.
König, A., K. Riedel, and J. W. Metzger (1998) A microbial sensor for detecting inhibitors of nitrification in wastewater. Biosens. Bioelectron. 13: 869–874.
König, A., T. T. Bachmann, J. W. Metzger, and R. D. Schmid (1999) Disposable sensor for measuring the biochemical oxygen demand for nitrification and inhibition of nitrification in wastewater. Appl. Microbiol. Biotechnol. 51: 112–117.
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Raud, M., Lember, E., Jõgi, E. et al. Nitrosomonas sp. Based biosensor for ammonium nitrogen measurement in wastewater. Biotechnol Bioproc E 18, 1016–1021 (2013). https://doi.org/10.1007/s12257-013-0078-x
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DOI: https://doi.org/10.1007/s12257-013-0078-x