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Improvement of the steroid dihydroxylation efficiency from dehydroepiandrosterone using a substrate pre-induction biotransformation process

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Abstract

This study investigated the effects of hydroxylase cyptochrome P450 inducers on the efficiency of the biotransformation of dehydroepiandrosterone (DHEA) to 3β, 7α, 15α-trihydroxy-5-androsten-17-one (7α,15α-diOHDHEA) by Colletotrichum lini ST-1. Special attention was given to the substrate DHEA being the best inducer and the fact that it could improve the yield of 7α, 15α-diOHDHEA. Based on the effects of the DHEA pre-induction phases and additional concentrations on 7α, 15α-diOHDHEA production, a substrate pre-induction process was established as follows: 2 g/L DHEA was added for the first time after 12 h of inoculation, followed by the second addition of 6 g/L DHEA after 12 h later. The results showed that this substrate pre-induction process improved the content of cytochrome P450 and that the 7α, 15α-diOH-DHEA yield reached 90.1%, which was 26.9% higher than that obtained in the original process.

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Authors and Affiliations

  1. School of Pharmaceutical Science, Jiangnan University, Wuxi, 214-122, China

    Hui Li, Heng Li, Wenfang Dou, Jinsong Shi & Zhenghong Xu

  2. School of Biotechnology, Jiangnan University, Wuxi, 214-122, China

    Zhenzhen Fu

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  1. Hui Li
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  2. Zhenzhen Fu
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  3. Heng Li
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  4. Wenfang Dou
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  5. Jinsong Shi
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  6. Zhenghong Xu
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Correspondence to Zhenghong Xu.

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These authors had equal contributions to this work.

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Li, H., Fu, Z., Li, H. et al. Improvement of the steroid dihydroxylation efficiency from dehydroepiandrosterone using a substrate pre-induction biotransformation process. Biotechnol Bioproc E 18, 486–490 (2013). https://doi.org/10.1007/s12257-012-0828-1

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  • DOI: https://doi.org/10.1007/s12257-012-0828-1

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