Skip to main content
SpringerLink
Log in

Growth and triterpenic acid accumulation of Cyclocarya paliurus cell suspension cultures

  • Research Paper
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Cyclocarya paliurus is a unique plant growing in central China with hypoglycaemic and hypolipaemia effects. To make better use of this functional food resource, cell suspension cultures and triterpenic acid accumulation were studied. Stable and uniform cell suspension cultures were established in liquid basal Murashige and Skoog medium supplemented with 2,4-dichlorophenoxy acetic acid (0.5 mg/L), naphthalene acetic acid (0.3 mg/L) and cytokinin (1.0 mg/L). According to the growth curve and triterpenic acid accumulation curve, the 8 ∼ 10th day postinoculation was the optimum time for subculture, and the 14th day was the optimum time for harvest. Murashige and Skoog medium and woody plant medium were suitable for both cell growth and triterpenic acid accumulation. 3% sucrose (w/v), 60 mM total nitrogen (NO3 −/NH4 + = 2/1), 1.25 mM KH2PO4, 2 mM CaCl2, and 2 mM MgSO4 were all found to be fit for cell growth and triterpenic acid accumulation in a cell suspension culture of Cyclocarya paliurus. Total triterpenic acid, ursolic acid and oleanolic acid content in suspended cultured cells were all significantly higher than that of leaves and calluses (P ⩽ 0.01), with levels up to 6.24, 2.28, and 0.94% (of dry weight), respectively. The betulinic acid content of suspended cultured cells also reached 0.82%, which was significantly higher than that of calluses. These results suggest that suspended cultured cells of Cyclocarya paliurus were rich in triterpenic acids and could be used for the production of total triterpenic acid, ursolic acid, oleanolic acid and betulinic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Xie, M. Y. and J. H. Xie (2008) Review about the research on Cyclocarya paliurus (Batal.) Iljinskaja. J. Food Sci. Biotechnol. 27: 113–121.

    Google Scholar 

  2. Yin, Z. P., X. C. Shangguan, D. M. Li, S. F. Wu, J. G. Chen, and Y. H. Zhang (2010) A study on ultrasonic-assisted extraction of total triterpenoids from Cyclocarya paliurus leaves. Acta Agriculturae Universitati 32: 373–377.

    CAS  Google Scholar 

  3. Fang, S. Z., W. X. Yang, X. L. Chu, X. L. Shang, C. Q. She, and X. X. Fu (2011) Provenance and temporal variations in selected flavonoids in leaves of Cyclocarya paliurus. Food Chem. 124: 1382–1386.

    Article  CAS  Google Scholar 

  4. Xie, J. H., M. Y. Xie, S. P. Nie, M. Y. Shen, Y. X. Wang, and C. Li (2010) Isolation, chemical composition and antioxidant activities of a water-soluble polysaccharide from Cyclocarya paliurus (Batal.) Iljinskaja. Food Chem. 119: 1626–1632.

    Article  CAS  Google Scholar 

  5. Fang, S. Z., X. L. Chu, X. L. Shang, W. X. Yang, X. X. Fu, and C. Q. She (2011) Seasonal variation of microelement contents in leaves of Cyclocarya paliurus among the provenances. J. Forest Res. 22: 225–231.

    Article  CAS  Google Scholar 

  6. Xie, M. Y., L. Li, S. P. Nie, X. R. Wang, and Frank S. C. Lee (2006) Determination of speciation of elements related to blood sugar in bioactive extracts from Cyclocarya paliurus leaves by FIA-ICP-MS. Eur. Food Res. Technol. 223: 202–209.

    Article  CAS  Google Scholar 

  7. Li, S., J. Li, X. L. Guan, J. Li, M. T. Deng, J. G. Huang, Z. Z. Chen, and R. Y. Yang (2011) Hypoglycemic effects and constituents of the barks of Cyclocarya paliurus and their inhibiting activities to glucosidase and glycogen phosphorylase. Fitoterapia 82: 1081–1085.

    Article  CAS  Google Scholar 

  8. Zhang, J., Q. Shen, J. C. Lu, J. Y. Li, W. Y. Liu, J. J. Yang, J. Li, and K. Xiao (2010) Phenolic compounds from the leaves of Cyclocarya paliurus (Batal.) Ijinskaja and their inhibitory activity against PTP1B. Food Chem. 119: 1491–1496.

    Article  CAS  Google Scholar 

  9. Kurihara, H., H. Fukami, A. Kusumoto, Y. Toyoda, H. Shibata, Y. Matsui, S. Asami, and T. Tanaka (2003) Hypoglycemic action of Cyclocarya paliurus (Batal.) Iljinskaja in normal and diabetic mice. Biosci. Biotechnol. Biochem. 67: 877–880.

    Article  CAS  Google Scholar 

  10. Kurihara, H., S. Asami, H. Shibata, H. Fukami, and T. Tanaka (2003) Hypolipemic effect of Cyclocarya paliurus (Batal.) Iljinskaja in lipid-loaded mice. Biol. Pharm. Bull. 26: 383–385.

    Article  CAS  Google Scholar 

  11. Xie, J. H., M.Y. Shen, M. Y. Xie, S. P. Nie, Y. Chen, C. Li, D. F. Huang, and Y. X. Wang (2012) Ultrasonic-assisted extraction, antimicrobial and antioxidant activities of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides. Carbohyd. Polym. 89: 177–184.

    Article  CAS  Google Scholar 

  12. Shu, R. G., C. R. Xu, L. N. Li, and Z. L. Yu (1995) Cyclocariosides II and III: Two secodammarane triterpenoid saponins from Cyclocarya paliurus. Planta Med. 61: 551–553.

    Article  CAS  Google Scholar 

  13. Li, S. A., B. S. Cui, Q. Liu, L. Tang, Y. C. Yang, X. J. Jin, and Z. F. Shen (2012) New triterpenoids from the leaves of Cyclocarya paliurus. Planta Med. 78: 290–296.

    Article  CAS  Google Scholar 

  14. Messner, B., I. Zeller, C. Ploner, S. Frotschnig, T. Ringer, A. Steinacher-Nigisch, A. Ritsch, G. Laufer, C. Huck, and D. Bernhard (2011) Ursolicacid causes DNA-damage, P53-mediated, mitochondria- and caspase-dependent human endothelial cell apoptosis, and accelerates atherosclerotic plaque formation in vivo. Atherosclerosis 219: 402–408.

    Article  CAS  Google Scholar 

  15. Buus, N. H., N. C. Hansson, R. Rodriguez-Rodriguez, E. Stankevicius, M. R. Anderson, and U. Simonsen (2011) Antiatherogenic effects of oleanolicacid in apolipoprotein E knockout mice. Eur. J. Pharmacol. 670: 519–526.

    Article  CAS  Google Scholar 

  16. Ghosh, J., J. Das, P. Manna, and P. C. Sil (2011) The protective role of arjunolic acid against doxorubicin induced intracellular ROS dependent JNK-p38 and p53-mediated cardiac apoptosis. Biomaterials 32: 4857–4866.

    Article  CAS  Google Scholar 

  17. Fang, S. Z., J. Y. Wang, Z. Y. Wei, and Z. X. Zhu (2006) Methods to break seed dormancy in Cyclocarya paliurus (Batal) Iljinskaja. Scientia Horticult. 110: 305–309.

    Article  CAS  Google Scholar 

  18. Georgiev, M. I., J. Weber, and A. Maciuk (2009) Bioprocessing of plant cell cultures for mass production of targeted compounds. Appl. Microbiol. Biotechnol. 83: 809–823.

    Article  CAS  Google Scholar 

  19. Ramachandra Rao, S. and G. A. Ravishankar (2002) Plant cell cultures: Chemical factories of secondary metabolites. Biotech. Adv. 20: 101–153.

    Article  CAS  Google Scholar 

  20. Bonfill, M., S. Mangas, E. Moyano, R. M. Cusido, and J. P. Palazon (2011) Production of centellosides and phytosterols in cell suspension cultures of Centella asiatica. Plant Cell Tiss. Organ. Cult. 104: 61–67.

    Article  CAS  Google Scholar 

  21. Nagella, P. and H. N. Murthy (2011) Effects of macroelements and nitrogen source on biomass accumulation and withanolide-A production from cell suspension cultures of Withania somnifera (L.) Dunal. Plant Cell Tiss. Organ. Cult. 104: 119–124.

    Article  CAS  Google Scholar 

  22. Srivastava, P., N. Kasoju, U. Bora, and R. Chaturvedi (2010) Accumulation of betulinic, oleanolic, and ursolic acids in in vitro cell cultures of Lantana camara L. and their significant cytotoxic effects on heLa cell lines. Biotechnol. Bioproc. Eng. 15: 1038–1046.

    Article  CAS  Google Scholar 

  23. Srivastava, P., V. Sisodia, and R. Chaturvedi (2011) Effect of cul- ture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L. Bioproc. Biosyst. Eng. 34: 75–80.

    Article  CAS  Google Scholar 

  24. Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacoo tissue cultures. Physiol. Plant 15: 473–497.

    Article  CAS  Google Scholar 

  25. Kolewe, M. E., M. A. Henson, and S. C. Roberts (2010) Characterization of aggregate size in Taxus suspension cell culture. Plant Cell Rep. 29: 485–494.

    Article  CAS  Google Scholar 

  26. Forni, C., A. Frattarelli, and C. Damiano (1999) Different size, shape and growth behavior of cells in suspension cultures of strawberry (Fragaria × ananassa Duch.). Plant Biosyst. 133: 205–212.

    Article  Google Scholar 

  27. Edahiro, J. and M. Seki (2006) Phenylpropanoid metabolite supports cell aggregate formation in strawberry cell suspension culture. J. Biosci. Bioeng. 102: 8–13.

    Article  CAS  Google Scholar 

  28. Boonsnongcheep, P., S. Korsangruang, N. Soonthornchareonnon, Y. Chintapakorn, P. Saralamp, and S. Prathanturarug (2010) Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tiss. Organ Cult. 101: 119–126.

    Article  CAS  Google Scholar 

  29. Yang, Y., F. He, and L. J. Yu (2008) Dynamics analyses of nutrients consumption and flavonoids accumulation in cell suspension culture of Glycyrrhiza inflata. Biol. Plantarum 52: 732–734.

    Article  CAS  Google Scholar 

  30. Nagella, P. and H. N. Murthy (2010) Establishment of cell suspension cultures of Withania somnifera for the production of withanolide A. Bioresource Technol. 101: 6735–6739.

    Article  CAS  Google Scholar 

  31. Simões, C., L. Silva Cordeiro, T. C. Castro, C. H. Callado, N. Albarello, and E. Mansur (2011) Establishment of anthocyaninproducing cell suspension cultures of Cleome rosea Vahl ex DC. (Capparaceae). Plant Cell Tiss. Organ Cult. 106: 537–545.

    Article  Google Scholar 

  32. Lian, M. L., D. Chakrabarty, and K. Y. Paek (2002) Effect of plant growth regulators and medium composition on cell growth and saponin production during cell suspension culture of mountain ginseng (Panax ginseng C.A. Mayer). J. Plant Biol. 45: 201–206.

    Article  CAS  Google Scholar 

  33. Naik, P. M., S. H. Manohar, and H. N. Murthy (2011) Effects of macro elements and nitrogen source on biomass accumulatin and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.). Acta Physiol. Plant 33: 1553–1557.

    Article  CAS  Google Scholar 

  34. Prakash, G. and A. K. Srivastava (2005) Statistical media optimization for cell growth and azadirachtin production in Azadirachta indica (A. Juss) suspension cultures. Proc. Biochem. 40: 3795–3800.

    Article  CAS  Google Scholar 

  35. Pan, X. W., H. H. Xu, X. Liu, X. Gao, and Y. T. Lu (2004) Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata. Biotechnol. Lett. 26: 1745–1748.

    Article  CAS  Google Scholar 

  36. Cordts, J. M., R. Scorza, and R. L. Bell (1987) Effects of carbohydrates and nitrogen on the development of anthocyanins of a red leaf peach (Prunus persica (L.) Batsch) in vitro. Plant Cell Tiss. Organ Cult. 9: 103–110.

    Article  CAS  Google Scholar 

  37. Yamada, J., K. Fujita, K. Eto, and K. Sakai (2003) Cell growth and nutrient uptake by cell suspensions of Cupressus lusitanica. J. Wood Sci. 49: 5–10.

    Article  CAS  Google Scholar 

  38. Shin, K. S., D. Chakrabarty, J. Y. Ko, S. S. Han, and Y. K. Paek (2003) Sucrose utilization and mineral nutrient uptake during hairy root growth of red beet (Beta vulgaris L.) in liquid culture. Plant Growth Regul. 39: 187–193.

    Article  CAS  Google Scholar 

  39. Yu, K. W., W. Y. Gao, E. J. Hahn, and K. Y. Paek (2001) Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C. A. Meyer). J. Plant Biol. 44: 179–184.

    Article  CAS  Google Scholar 

  40. Weathers, P. J., M. J. Towler, and J. F. Xu (2010) Bench to batch: Advances in plant cell culture for producing useful products. Appl. Microbiol. Biotechnol. 85: 1339–1351.

    Article  CAS  Google Scholar 

  41. Huang, T. K. and K. A. McDonald (2009) Bioreactor engineering for recombinant protein production in plant cell suspension cultures. Biochem. Eng. J. 45: 168–184.

    Article  CAS  Google Scholar 

  42. Smetanska, I. (2008) Production of secondary metabolites using plant cell cultures. Adv. Biochem. Eng. Biotechnol. 111: 187–228.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Jiangxi Key Laboratory of Natural Products and Functional food, Jiangxi Agricultural University, Nanchang, 330-045, China

    Zhongping Yin, Xinchen Shangguan, Jiguang Chen & Dongming Li

  2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330-047, China

    Qiang Zhao

Authors
  1. Zhongping Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xinchen Shangguan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jiguang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qiang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dongming Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xinchen Shangguan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yin, Z., Shangguan, X., Chen, J. et al. Growth and triterpenic acid accumulation of Cyclocarya paliurus cell suspension cultures. Biotechnol Bioproc E 18, 606–614 (2013). https://doi.org/10.1007/s12257-012-0751-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-012-0751-5

Keywords

Search

Navigation