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Enzymatic synthesis of fructooligosaccharides from date by-products using an immobilized crude enzyme preparation of β-D-fructofuranosidase from Aspergillus awamori NBRC 4033

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Abstract

Aqueous extracts from date by-products of the sucrose-rich variety “Deglet Nour” were used as a starting substrate to achieve the enzymatic synthesis of fructooligosaccharides (FOS) commonly used as prebiotics. A crude β-fructofuranosidase (Ffase) preparation from Aspergillus awamori NBRC4033 was immobilized on chitosan by covalent binding through glutaraldehyde linkages (Yi = 88%, Ya = 54%), and used for this purpose. The effect of water-extraction volume on the FOS synthesis by transfructosylation was studied. It was found that 150 mL/100 g of date by-products gave the best FOS concentration and productivity (123 g/L and 18.5 g/h/100 g respectively), related to an optimal sucrose conversion of 53.26%. The main FOS product was purified via a biogel-P2 gel filtration column. Its structure was determined as 1-kestose: α-Dglucopyranosyl-( 1→2)-β-D-fructofuranosyl-(2→1)-β-Dfructofuranoside by combination of 1H, 13C and 2D-NMR techniques. Our results provide new insights into the enzymatic synthesis of FOS from an alternative source of sucrose, namely date by-products.

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Authors and Affiliations

  1. Laboratory of Protein and Bioactive Molecules Engineering-INSAT, Centre Urbain Nord, University of Carthage, BP 676, 1080, Tunis cedex, Tunisia

    Issam Smaali, Souhir Jazzar, Asma Soussi & M. Nejib Marzouki

  2. Institut de chimie moléculaire, UMR 6229, CNRS-URCA, URCA, 51687, Reims, France

    Murielle Muzard

  3. INRA-URCA UMR FARE 614. 8, rue Gabriel Voisin, BP 316, F51688, Reims Cedex 2, France

    Nathalie Aubry

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  1. Issam Smaali
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  2. Souhir Jazzar
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  3. Asma Soussi
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  4. Murielle Muzard
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  6. M. Nejib Marzouki
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Correspondence to Issam Smaali.

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Smaali, I., Jazzar, S., Soussi, A. et al. Enzymatic synthesis of fructooligosaccharides from date by-products using an immobilized crude enzyme preparation of β-D-fructofuranosidase from Aspergillus awamori NBRC 4033. Biotechnol Bioproc E 17, 385–392 (2012). https://doi.org/10.1007/s12257-011-0388-9

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  • DOI: https://doi.org/10.1007/s12257-011-0388-9

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