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Influence of apoproteins for enediyne production

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Abstract

Kedarcidin and neocarzinostatin belong to the chromoprotein family of endiyene antibiotics and have potent antitumor activity. Their structures consist of an apoprotein and a nonpeptide chromophore. Apoprotein has been known to be essential to stabilize and regulate the availability of a labile chromophore. Here, we have identified the complete kedarcidin apoprotein gene (kedA) from Streptoalloteichus sp. ATCC 53650 (Actinomycete L585-6). KedA was characterized by a comparative study with neocarzinostatin apoprotein (NcsA). Overexpression of kedA resulted in a ∼ 2-fold enhanced production of kedarcidin in Streptoalloteichus sp., whereas overexpression of ncsA resulted in a ∼ 1.6-fold enhanced production of neocarzinostatin in Streptomyces carzinostaticus ATCC 15944. Moreover, the apoprotein-overexpressed strain showed a high growth rate, which demonstrates that the apoprotein not only protects the labile chromophore, but also tolerates the toxicity of endiyene antibiotics in the host strain.

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Asan, 336-708, Korea

    Vu Thi Thu Hang, Tae Su Kim, Tae-Jin Oh & Jae Kyung Sohng

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  1. Vu Thi Thu Hang
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  2. Tae Su Kim
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  3. Tae-Jin Oh
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  4. Jae Kyung Sohng
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Correspondence to Jae Kyung Sohng.

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First two authors contributed equally to this work.

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Hang, V.T.T., Kim, T.S., Oh, TJ. et al. Influence of apoproteins for enediyne production. Biotechnol Bioproc E 16, 462–469 (2011). https://doi.org/10.1007/s12257-010-0369-4

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  • DOI: https://doi.org/10.1007/s12257-010-0369-4

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