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Dilute acid pretreatment of barley straw and its saccharification and fermentation

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Abstract

In this study, the optimization of the major factors for efficient dilute acid pretreatment (DAP) of Korean barley straw was conducted by response surface method (RSM). In addition, saccharification of the optimized pretreated barley straw as well as fermentation of solubilized hemicellulose and enzymatic hydrolysates was performed for bioethanol production. The factors optimized by RSM were concentration of sulfuric acid, reaction time and temperature. Optimization experiments were carried out within the scope of 0.16 ∼ 1.84% sulfuric acid, 10 ∼ 20 min of reaction time, and 116 ∼ 183°C of temperature using a statistical program, and optimal conditions (1.16% of sulfuric acid, 16.9 min of reaction time, and 150°C) were determined based on reliable statistical indicators. The predicted value at stationary point and the experimental value were 81.38 and 80.66%, respectively. Saccharification was performed at 50°C using Celluclast (cellulase) and Novozyme 188 (β-glucosidase) as biocatalysts in an enzyme loading test. Conversion of the saccharification process was approximately 65%. In addition, fermentation of glucose after saccharification and solubilization of xylose solution by DAP were performed using Saccharomyces cerevisiae and Pichia stipitis at 30°C and 200 rpm for 12 h.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, Seoul, 136-701, Korea

    Sung Bong Kim, Ja Hyun Lee, Sang Jun Lee, Jun Seok Kim & Seung Wook Kim

  2. Department of Chemical Engineering, Dankuk University, Chonan, 139-701, Korea

    Kyeong Keun Oh

  3. Department of Chemical Engineering, Kyonggi University, Suwon, 443-760, Korea

    Jin Young Lee

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  1. Sung Bong Kim
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  2. Ja Hyun Lee
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  3. Kyeong Keun Oh
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  4. Sang Jun Lee
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  5. Jin Young Lee
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Correspondence to Seung Wook Kim.

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Kim, S.B., Lee, J.H., Oh, K.K. et al. Dilute acid pretreatment of barley straw and its saccharification and fermentation. Biotechnol Bioproc E 16, 725–732 (2011). https://doi.org/10.1007/s12257-010-0305-7

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  • DOI: https://doi.org/10.1007/s12257-010-0305-7

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