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Aeration alleviates ethanol inhibition and glycerol production during fed-batch ethanol fermentation

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Abstract

In this study, we investigated the effects of aeration on ethanol inhibition and glycerol production during fed-batch ethanol fermentation. When aeration was conducted at 0.13, 0.33, and 0.8 vvm, the ethanol productivity, specific ethanol production rate, and ethanol yield in the presence of greater than 100 g/L of ethanol were higher than when aeration was not conducted. In addition, estimation of the parameters (α and β) in a model equation of ethanol inhibition kinetics indicated that aeration alleviated ethanol inhibition against the specific growth rate and the specific ethanol production rate. Specifically, when aeration was conducted, the glycerol yield and specific glycerol production rate decreased approximately 50 and 70%, respectively. Finally, the results of this study indicated that aeration during fed-batch ethanol fermentation may improve the ethanol concentration in the final culture broth, as well as the ethanol productivity.

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Authors and Affiliations

  1. Department of Biotechnology, Chungju National University, Chungbuk, 368-701, Korea

    Hyeon-Beom Seo, Ji-Hyeon Yeon & Kyung-Hwan Jung

  2. Division of Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Korea

    Myung Hoon Jeong & Hyeon-Yong Lee

  3. Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Korea

    Hyeon-Yong Lee

  4. Korea Ocean Research & Development Institute, Ansan, 426-744, Korea

    Do Hyung Kang

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  1. Hyeon-Beom Seo
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Correspondence to Kyung-Hwan Jung.

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Seo, HB., Yeon, JH., Jeong, M.H. et al. Aeration alleviates ethanol inhibition and glycerol production during fed-batch ethanol fermentation. Biotechnol Bioproc E 14, 599–605 (2009). https://doi.org/10.1007/s12257-009-0066-3

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  • DOI: https://doi.org/10.1007/s12257-009-0066-3

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