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High-level production of ethanol during fed-batch ethanol fermentation with a controlled aeration rate and non-sterile glucose powder feeding of Saccharomyces cerevisiae

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Abstract

In this study, we utilized a unique strategy for fed-batch fermentation using ethanol-tolerant Saccharomyces cerevisiae to achieve a high-level of ethanol production that could be practically applied on an industrial scale. During this study, the aeration rate was controlled at 0.0, 0.13, 0.33, and 0.8 vvm to determine the optimal aeration conditions for the production of ethanol. Additionally, non-sterile glucose powder was fed during fed-batch ethanol fermentation and corn-steep liquor (CSL) in the medium was used as an organic N-source. When aeration was conducted, the ethanol production and productivity were superior to that when aeration was not conducted. Specifically, the maximum ethanol production reached approximately 160 g/L, when the fermentor was aerated at 0.13 vvm. These findings indicate that the use of a much less expensive C-source may enable the fermentation process to be directed towards the improvement of overall ethanol production and productivity in fermentors that are aerated at 0.13 vvm. Furthermore, if a repeated fed-batch process in which the withdrawal and fill is conducted prior to 36 h can be employed, aeration at a rate of 0.33 and/or 0.8 vvm may improve the overall ethanol productivity

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Authors and Affiliations

  1. Department of Biotechnology, Chungju National University, Chungbuk, 368-701, Korea

    Hyeon-Beom Seo & Kyung-Hwan Jung

  2. Division of Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Korea

    Seung Seop Kim & Hyeon-Yong Lee

  3. Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Korea

    Hyeon-Yong Lee

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  1. Hyeon-Beom Seo
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  2. Seung Seop Kim
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  4. Kyung-Hwan Jung
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Correspondence to Kyung-Hwan Jung.

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Seo, HB., Kim, S.S., Lee, HY. et al. High-level production of ethanol during fed-batch ethanol fermentation with a controlled aeration rate and non-sterile glucose powder feeding of Saccharomyces cerevisiae . Biotechnol Bioproc E 14, 591–598 (2009). https://doi.org/10.1007/s12257-008-0274-2

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