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Optimization of human interferon gamma production in Escherichia coli by response surface methodology

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Abstract

The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, bio-mass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a second order response function was developed. The optimal expression conditions were a temperature of 32.6°C, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized conditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli.

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Authors and Affiliations

  1. Instituto Potosino de Investigación Científica y Tecnológica, División de Biología Molecular, Camino a la Presa San José 2055 Col. Lomas 4a sección, CP 78216, San Luis Potosí, S.L.P., México

    Victor E. Balderas Hernández, Luz M. T. Paz Maldonado, Emilio Medina Rivero, Ana P. Barba de la Rosa, Leandro G. Ordoñez Acevedo & Antonio De León Rodríguez

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  1. Victor E. Balderas Hernández
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  2. Luz M. T. Paz Maldonado
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  3. Emilio Medina Rivero
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  4. Ana P. Barba de la Rosa
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  5. Leandro G. Ordoñez Acevedo
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  6. Antonio De León Rodríguez
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Correspondence to Antonio De León Rodríguez.

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Balderas Hernández, V.E., Paz Maldonado, L.M.T., Medina Rivero, E. et al. Optimization of human interferon gamma production in Escherichia coli by response surface methodology. Biotechnol Bioproc E 13, 7–13 (2008). https://doi.org/10.1007/s12257-007-0126-5

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  • DOI: https://doi.org/10.1007/s12257-007-0126-5

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