Abstract
XPC gene belongs to DNA repair pathway, which is involved in the development of uterine leiomyoma. However, its relationships with leiomyoma risk were never reported. We here hypothesized that XPC gene was associated with the risk of uterine leiomyoma. In this case–control study with a total of 391 leiomyoma cases and 493 tumor-free controls in a reproductive women population in South China, two missense polymorphisms rs2228001 A > C (Lys939Gln) and rs2228000 C > T (Ala499Val) were genotyped by quantitative polymerase chain reaction (qPCR). Then, the associations between these two polymorphisms and leiomyoma risk were investigated. It was revealed that the rs2228000 CT/TT variant genotypes had a decreased leiomyoma risk (adjusted odds ratio = 0.73, 95% confidence interval = 0.54–0.94) compared with rs2228000 CC genotype. Further stratified analysis also revealed that the protective effect of rs2228000 CT/TT on the risk of uterine leiomyoma was more evident among subjects who were younger than 35 years old compared with those with larger tumors (diameter of tumor >5 cm), and those with fewer number of myomas (only one). However, no significant association was observed for leiomyoma risk for rs2228001 A > C. This study indicated that genetic variations in XPC gene are associated with leiomyoma susceptibility in a reproductive women population. It warrants further confirmation in larger prospective studies with different populations.
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Acknowledgements
This study was partially supported by Sanming Project of Medicine in Shenzhen (No.:SZSM201406007). We thank Drs. Sun, Chen, Guan, Chi, Jian, Zhu, and Liu for sample collecting and histological examinations.
Funding
This work was partially supported by Sanming Project of Medicine in Shenzhen (No.:SZSM201406007).
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Conceived and designed the experiments: BL. Performed the experiments: ZL and ML. Collected samples: ML. Analyzed data: ZL and BL. Contributed to the writing of manuscript: ZL and BL. All authors approved the final manuscript.
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All the study participants gave a written informed consent. A formal consent was also issued by the Ethics Committee of the Baoan Maternal and Child Health Hospital, Jinan University (No. of Institutional Review Board: LLSC2018-02-01).
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Liu, ZQ., Lu, MY. & Liu, B. Polymorphisms in XPC Gene and Risk of Uterine Leiomyoma in Reproductive Women. Pathol. Oncol. Res. 26, 1459–1464 (2020). https://doi.org/10.1007/s12253-019-00720-2
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DOI: https://doi.org/10.1007/s12253-019-00720-2