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The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade

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Pathology & Oncology Research

Abstract

Here, we report the p53 dependent mitochondria-mediated apoptotic mechanism of plant derived silver-nanoparticle (PD-AgNPs) in colorectal cancer cells (CRCs). PD-AgNPs was synthesized by reduction of AgNO3 with leaf extract of a medicinal plant periwinkle and characterized. Uptake of PD-AgNPs (ξ - 2.52 ± 4.31 mV) in HCT116 cells was 3 fold higher in comparison to synthetic AgNPs (ξ +2.293 ± 5.1 mV). A dose dependent increase in ROS production, activated JNK and decreased mitochondrial membrane potential (MMP) were noted in HCT116 but not in HCT116 p53 −/− cells after PD-AgNP exposure. PD-AgNP-mediated apoptosis in CRCs is a p53 dependent process involving ROS and JNK cascade.

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Acknowledgments

Authors are greatly acknowledged Prof. Bert Vogelstein, Johns Hopkins, Baltimore, USA for providing HCT116 p53 −/− cell lines and also thanks to ICMR and DBT, Govt. of India for financial support.

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa, 751024, India

    Shakti Ranjan Satapathy, Purusottam Mohapatra, Dipon Das, Sumit Siddharth & Chanakya Nath Kundu

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  1. Shakti Ranjan Satapathy
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  2. Purusottam Mohapatra
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  3. Dipon Das
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  4. Sumit Siddharth
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  5. Chanakya Nath Kundu
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Correspondence to Chanakya Nath Kundu.

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Satapathy, S.R., Mohapatra, P., Das, D. et al. The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade. Pathol. Oncol. Res. 21, 405–411 (2015). https://doi.org/10.1007/s12253-014-9835-1

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