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Analysis of hsa-miR-30a-5p Expression in Human Gliomas

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Pathology & Oncology Research

Abstract

Our previous study demonstrated that miR-30a-5p was upregulated in six malignant glioma cell lines by microRNA(miRNA) array. For further verification of this finding, the expression of miR-30a-5p in 7 more malignant glioma cell lines, 43 freshly resected glioma samples and 75 archival paraffin embedded glioma specimens with different grade of malignancy were examined by qRT-PCR and in situ hybridization(ISH). Here, we present the first evidence that miR-30a-5p is overexpressed in glioma cell lines and glioma samples as compared to the normal brain tissues (NBTs), and its expression level is positively correlated with tumor grade of malignancy. It is concluded that miR-30a-5p may have the potential as a diagnostic or prognostic marker of gliomas and as the target of miRNA-based glioma therapy in further studies.

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Authors and Affiliations

  1. Department of Neurosurgery, Hangzhou Xiasha Hospital, Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou, 310016, People’s Republic of China

    Kun Wang

  2. Department of Neurosurgery, Tianjin Medical University General Hospital, 152 An-Shan Road, Heping District, Tianjin, 300052, People’s Republic of China

    Zhifan Jia, Anling Zhang, Guangxiu Wang, Jianwei Hao & Peiyu Pu

  3. Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, 300052, People’s Republic of China

    Zhifan Jia, Anling Zhang, Guangxiu Wang, Jianwei Hao & Peiyu Pu

  4. Women’s Reproductive Health Laboratory of Zhejiang Province, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, People’s Republic of China

    Jian Zou

  5. Department of Neurosurgery, Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou, 310016, People’s Republic of China

    Yirong Wang & Shuxu Yang

Authors
  1. Kun Wang
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  2. Zhifan Jia
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  3. Jian Zou
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  4. Anling Zhang
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  5. Guangxiu Wang
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  6. Jianwei Hao
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  7. Yirong Wang
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  8. Shuxu Yang
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  9. Peiyu Pu
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Corresponding author

Correspondence to Peiyu Pu.

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Kun Wang and Zhifan Jia contribute equally to the present work.

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Wang, K., Jia, Z., Zou, J. et al. Analysis of hsa-miR-30a-5p Expression in Human Gliomas. Pathol. Oncol. Res. 19, 405–411 (2013). https://doi.org/10.1007/s12253-012-9593-x

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  • DOI: https://doi.org/10.1007/s12253-012-9593-x

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