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Human Papillomavirus DNA and Oncogene Alterations in Colorectal Tumors

  • Published:
Pathology & Oncology Research

Abstract

The aim of the present study is to determine the presence and molecular integrity of high-risk HPV types in colorectal adenocarcinomas and to assess whether viral DNA is related to common proto-oncogene alterations, such as k-ras mutations and c-myc gene amplification, in colorectal cancer. Seventy-five colorectal adenocarcinomas were screened for HPV infection using nested-PCR (MY09/11-GP5+/6+). HPV typing was performed by type-specific PCR for HPV 16 and HPV 18 DNA. Unidentified samples were subsequently sequenced to determine the viral genotype. The physical status of HPV was determined by a nested PCR approach for type-specific E2 sequences. C-myc amplification was assessed by co-amplification with β-globin as control locus, and mutation in k-ras codons 12 and 13 by ARMS-PCR. Overall, HPV was detected in thirty-three colorectal specimens (44%). HPV 16 was the prevalent type (16/75), followed by HPV 18 (15/75), HPV 31 (1/75) and HPV 66 (1/75). E2 disruption was detected in 56.3% of HPV 16 and in 40% of HPV 18 positive tumors. C-myc amplification was detected in 29.4% of cases, while k-ras mutations in 30.7%. There was no significant trend for HPV infection in tumors harboring either k-ras or c-myc alterations. This study demonstrates HPV DNA and viral integration in colorectal tumors, suggesting a potential role of this virus in colorectal carcinogenesis. There was no concurrence, however, of k-ras and c-myc activation with viral infection.

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Acknowledgments

The authors would like to thank continuous support from IGEVET-CONICET and especially Dr. Silvina Díaz for technical support in cloning the HPV genomes. This work was partially funded by grant PICT 20149, ANPCyT.

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Authors and Affiliations

  1. IGEVET-CONICET (Institute of Veterinary Genetics-National Council of Scientific and Technical Investigations), National University of La Plata, 60 and 118 street, PC B1900, La Plata, Buenos Aires, Argentina

    Luis Orlando Pérez, Gisela Barbisan & Carlos Daniel Golijow

  2. Service of Pathology, Interzonal Hospital of San Martín, 1791 1st street, PC B1900, La Plata, Buenos Aires, Argentina

    Anabel Ottino

  3. Service of Pathology, Interzonal Hospital of San Martín, 1791 1st street, PC B1900, La Plata, Argentina

    Horacio Pianzola

  4. Faculty of Medical Sciences, Department of Pathology, National University of La Plata, 60 and 122 street, PC B1900, La Plata, Buenos Aires, Argentina

    Horacio Pianzola

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  1. Luis Orlando Pérez
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  2. Gisela Barbisan
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  3. Anabel Ottino
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  4. Horacio Pianzola
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  5. Carlos Daniel Golijow
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Correspondence to Luis Orlando Pérez.

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Pérez, L.O., Barbisan, G., Ottino, A. et al. Human Papillomavirus DNA and Oncogene Alterations in Colorectal Tumors. Pathol. Oncol. Res. 16, 461–468 (2010). https://doi.org/10.1007/s12253-010-9246-x

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  • DOI: https://doi.org/10.1007/s12253-010-9246-x

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