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Genome analysis of Heliothis virescens ascovirus 3h isolated from China

  • Research Article
  • Published:
Virologica Sinica

Abstract

No ascovirus isolated from China has been sequenced so far. Therefore, in this study, we aimed to sequence the genome of Heliothis virescens ascovirus 3h (HvAV-3h) using the 454 pyrosequencing technology. The genome was found to be 190,519-bp long with a G+C content of 45.5%. We also found that it encodes 185 hypothetical open reading frames (ORFs) along with at least 50 amino acids, including 181 ORFs found in other ascoviruses and 4 unique ORFs. Gene-parity plots and phylogenetic analysis revealed a close relationship between HvAV-3h and three other HvAV-3a strains and a distant relationship with Spodoptera frugiperda ascovirus 1a (SfAV-1a), Trichoplusia ni ascovirus 6a (TnAV-6a), and Diadromus pulchellus ascovirus 4a (DpAV-4a). Among the 185 potential genes encoded by the genome, 44 core genes were found in all the sequenced ascoviruses. In addition, 25 genes were found to be conserved in all ascoviruses except DpAV-4a. In the HvAV-3h genome, 24 baculovirus repeat ORFs (bros) were present, and the typical homologous repeat regions (hrs) were absent. This study supplies information important for understanding the conservation and functions of ascovirus genes as well as the variety of ascoviral genomes.

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Acknowledgments

The authors would like to thank Jue Hu, Xing-Shi Gu, Wen-Fei Xian, Hai-Zhou Liu, Lei Zhang, and Shun-Ji Li for their help in virus amplification and data analysis. The authors would also like to acknowledge the technical assistance received from the Core Facility and Technical Support of Wuhan Institute of Virology. This study was supported partly by the National Natural Science Foundation of China (No. 31371995 and 31621061), the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB11030400), STS Project of the Chinese Academy of Sciences (grant KFJ-SW-STS- 143-3), and the Hunan Provincial Natural Science Foundation for Distinguished Young Scholar of China (14JJ1023).

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Authors and Affiliations

  1. State Key Laboratory of Virology, Microorganisms & Viruses Culture Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China

    Guo-Hua Huang, Dian-Hai Hou, Manli Wang & Zhihong Hu

  2. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Institute of Virology, Hunan Agricultural University, Changsha, Hunan, 410128, China

    Guo-Hua Huang

  3. Department of Microbiology, 32 Pearson Hall, Miami University, Oxford, Ohio, 45056, USA

    Xiao-Wen Cheng

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  1. Guo-Hua Huang
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  2. Dian-Hai Hou
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  3. Manli Wang
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  4. Xiao-Wen Cheng
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  5. Zhihong Hu
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Correspondence to Zhihong Hu.

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ORCID: 0000-0002-1560-0928

These authors contributed equally to this work.

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Huang, GH., Hou, DH., Wang, M. et al. Genome analysis of Heliothis virescens ascovirus 3h isolated from China. Virol. Sin. 32, 147–154 (2017). https://doi.org/10.1007/s12250-016-3929-8

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