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Comparison of concentration methods for detection of hepatitis A virus in water samples

  • Research Article
  • Published:
Virologica Sinica

Abstract

Hepatitis A virus is a pathogen associated with water pollution. Contaminated drinking water can cause hepatitis A outbreaks, lead to economic losses, and even threaten human lives. It is difficult to detect low levels of hepatitis A virus in water, so the virus must be concentrated in order to quantify it accurately. Here, we present a simple, rapid, efficient technique for the concentration and detection of hepatitis A virus in water. Our data showed that adding phosphate-buffered saline to the water, pre-filtering the water, and adding Trizol reagent directly to the filtration membrane can significantly improve concentration efficiency. Of three types of filtration membranes studied (mixed cellulose ester membrane, polyvinylidene fluoride membrane, and nylon membrane), the concentration efficiency using mixed cellulose ester membrane with a 0.1-μm pore size was the highest, reaching 92.62 ± 5.17%. This method was used to concentrate hepatitis A virus in water samples from Donghu Lake. Using SYBR Green real-time reverse transcription polymerase chain reaction analysis, the detection sensitivity of this method reached 101 copies/μL and its concentration efficiency reached 79.45 ± 9.88%.

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References

  • Beji-Hamza A, Khelifi-Gharbi H, Hassine-Zaafrane M, Della Libera S, Iaconelli M, Muscillo M, Petricca S, Ciccaglione AR, Bruni R, Taffon S, Equestre M, Aouni M, La Rosa G. 2014. Qualitative and quantitative assessment of hepatitis A virus in wastewaters in Tunisia. Food Environ Virol, 6: 246–252.

    Article  CAS  PubMed  Google Scholar 

  • Brooks HA, Gersberg RM, Dhar AK. 2005. Detection and quantification of hepatitis A virus in seawater via real-time RT-PCR. J Virol Methods, 127: 109–118.

    Article  CAS  PubMed  Google Scholar 

  • Cohen JI, Ticehurst JR, Purcell RH, Buckler-White A, Baroudy BM. 1987. Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses. J Virol, 61: 50–59.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Collier MG, Khudyakov YE, Selvage D, Adams-Cameron M, Epson E, Cronquist A, Jervis RH, Lamba K, Kimura AC, Sowadsky R, Hassan R, Park SY, Garza E, Elliott AJ, Rotstein DS, Beal J, Kuntz T, Lance SE, Dreisch R, Wise ME, Nelson NP, Suryaprasad A, Drobeniuc J, Holmberg SD, Xu F, Hepatitis AOIT. 2014. Outbreak of hepatitis A in the USA associated with frozen pomegranate arils imported from Turkey: an epidemiological case study. Lancet Infect Dis, 14: 976–981.

    Article  PubMed  Google Scholar 

  • Costa-Mattioli M, Di Napoli A, Ferre V, Billaudel S, Perez-Bercoff R, Cristina J. 2003. Genetic variability of hepatitis A virus. J Gen Virol, 84: 3191–3201.

    Article  CAS  PubMed  Google Scholar 

  • Cuthbert JA. 2001. Hepatitis A: old and new. Clin Microbiol Rev, 14: 38–58.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • El Galil KHA, El Sokkary MA, Kheira SM, Salazar AM, Yates MV, Chen W, Mulchandani A. 2004. Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples. Appl Environ Microb, 70: 4371–4374.

    Article  Google Scholar 

  • Fong TT, Lipp EK. 2005. Enteric viruses of humans and animals in aquatic environments: health risks, detection, and potential water quality assessment tools. Microbiol Mol Biol Rev, 69: 357–371.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gazette of the Ministry of Health of People’s Republic of China. 2012). http://govinfo.nlc.gov.cn/gtfz/zfgb/wsb/688748a/201204/ P020120416401125621405.pdf?classid=373). (In Chinese)

  • Gupta E, Ballani N. 2014. State of the globe: hepatitis A virus - return of a water devil. J Glob Infect Dis, 6: 57–58.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Halliday ML, Kang LY, Zhou TK, Hu MD, Pan QC, Fu TY, Huang YS, Hu SL. 1991. An epidemic of hepatitis A attributable to the ingestion of raw clams in Shanghai, China. J Infect Dis, 164: 852–859.

    Article  CAS  PubMed  Google Scholar 

  • Haramoto E, Katayama H, Oguma K, Ohgaki S. 2007. Quantitative analysis of human enteric adenoviruses in aquatic environments. J Appl Microb, 103: 2153–2159.

    Article  CAS  Google Scholar 

  • Haramoto E, Katayama H, Ohgaki S. 2004. Detection of Noroviruses in tap water in Japan by means of a new method for concentrating enteric viruses in large volumes of freshwater. Appl Environ Microbiol, 70: 2154–2160.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hsu BM, Chen CH, Kung CM, Wan MT, Shen SM. 2007. Evaluation of enterovirus recovery in surface water by different adsorption and elution procedures. Chemosphere, 66: 964–969.

    Article  CAS  PubMed  Google Scholar 

  • Ikner LA, Gerba CP, Bright KR. 2012. Concentration and recovery of viruses from water: a comprehensive review. Food Environ Virol, 4: 41–67.

    Article  PubMed  Google Scholar 

  • Jaykus LA, de Leon R, Sobsey MD. 1996. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization. Appl Environ Microbiol, 62: 2074–2080.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang W, Zhuang L, Li S, Zhou J, Tang G, Wang D. 2013. Gene type analysis of hepatitis A virus in four outbreak cases in Guizhou province, China, from 2008 to 2009. Mod Prev Med, 40: 4260–4264. (In Chinese)

    CAS  Google Scholar 

  • Kittigul L, Ekchaloemkiet S, Utrarachkij F, Siripanichgon K, Sujirarat D, Pungchitton S, Boonthum A. 2005. An efficient virus concentration method and RT-nested PCR for detection of rotaviruses in environmental water samples. J Virol Methods, 124: 117–122.

    Article  CAS  PubMed  Google Scholar 

  • Kittigul L, Khamoun P, Sujirarat D, Utrarachkij F, Chitpirom K, Chaichantanakit N, Vathanophas K. 2001. An improved method for concentrating rotavirus from water samples. Memorias Do Instituto Oswaldo Cruz, 96: 815–821.

    Article  CAS  PubMed  Google Scholar 

  • Martin A, Lemon SM. 2006. Hepatitis A virus: from discovery to vaccines. Hepatology, 43: S164–S172.

    Article  CAS  PubMed  Google Scholar 

  • McCaustland KA, Bond WW, Bradley DW, Ebert JW, Maynard JE. 1982. Survival of hepatitis A virus in feces after drying and storage for 1 month. J Clin Microbiol, 16: 957–958.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Melnick JL. 1992. Properties and classification of hepatitis A virus. Vaccine, 10 Suppl 1: S24–S26.

    Article  PubMed  Google Scholar 

  • Monceyron C, Grinde B. 1994. Detection Of hepatitis-A virus in clinical and environmental-samples by immunomagnetic separation and PCR. J Virol Methods, 46: 157–166.

    Article  CAS  PubMed  Google Scholar 

  • Rzezutka A, Cook N. 2004. Survival of human enteric viruses in the environment and food. FEMS Microbiol Rev, 28: 441–453.

    Article  CAS  PubMed  Google Scholar 

  • Schwab KJ, Deleon R, Sobsey MD. 1995. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis-A virus, and Norwalk virus by Reverse Transcription-PCR. Appl Environ Microb, 61: 531–537.

    CAS  Google Scholar 

  • Villar LM, de Paula VS, Diniz-Mendes L, Lampe E, Gaspar AMC. 2006. Evaluation of methods used to concentrate and detect hepatitis A virus in water samples. J Virol Methods, 137: 169–176.

    Article  CAS  PubMed  Google Scholar 

  • Westrell, T., Teunis P, van den Berg H, Lodder W, Ketelaars H, Stenstrom TA, de Roda Husman AM. 2006. Short- and longterm variations of norovirus concentrations in the Meuse River during a 2-year study period. Water Res, 40: 2613–2620.

    Article  CAS  PubMed  Google Scholar 

  • Wang S. 2009. Master’s thesis. Huazhong University of Science and Technology, Wuhan, China. Study on the methods of membran adsorption-elution and immunomagnetic beads adsorption for concentrating virus in water.

    Google Scholar 

  • Zhang ML, Yang J, Zhao H, Zhu L, Zhao HB, Cai BL. 2009. Detection and quantification of hepatitis A virus in Tianjin coastal seawater of Bohai Bay. Environ Sci, 30: 1608–1613. (In Chinese)

    Google Scholar 

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Authors and Affiliations

  1. CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Yuting Qiao, Lihua Zhao & Quanjiao Chen

  2. National Institute of Metrology, Beijing, 100013, China

    Zhiwei Sui

  3. College of Animal Science and Technology, Institute of Animal Population Health, Jiangxi Agricultural University, Nanchang, 330045, China

    Yuting Qiao, Guoliang Hu & Huabin Cao

  4. Hubei Wildlife Epidemic Focus and Disease Surveillance Center, Wuhan, 430075, China

    Guoxiang Yang, Yong Li & Yongsong Lei

Authors
  1. Yuting Qiao
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  2. Zhiwei Sui
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  3. Guoliang Hu
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  4. Huabin Cao
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  5. Guoxiang Yang
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  6. Yong Li
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  7. Yongsong Lei
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  8. Lihua Zhao
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  9. Quanjiao Chen
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Corresponding author

Correspondence to Quanjiao Chen.

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ORCID: 0000-0001-8716-6510

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Qiao, Y., Sui, Z., Hu, G. et al. Comparison of concentration methods for detection of hepatitis A virus in water samples. Virol. Sin. 31, 331–338 (2016). https://doi.org/10.1007/s12250-016-3786-5

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  • DOI: https://doi.org/10.1007/s12250-016-3786-5

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