Virologica Sinica

, Volume 31, Issue 4, pp 331–338 | Cite as

Comparison of concentration methods for detection of hepatitis A virus in water samples

  • Yuting Qiao
  • Zhiwei Sui
  • Guoliang Hu
  • Huabin Cao
  • Guoxiang Yang
  • Yong Li
  • Yongsong Lei
  • Lihua Zhao
  • Quanjiao Chen
Research Article
  • 87 Downloads

Abstract

Hepatitis A virus is a pathogen associated with water pollution. Contaminated drinking water can cause hepatitis A outbreaks, lead to economic losses, and even threaten human lives. It is difficult to detect low levels of hepatitis A virus in water, so the virus must be concentrated in order to quantify it accurately. Here, we present a simple, rapid, efficient technique for the concentration and detection of hepatitis A virus in water. Our data showed that adding phosphate-buffered saline to the water, pre-filtering the water, and adding Trizol reagent directly to the filtration membrane can significantly improve concentration efficiency. Of three types of filtration membranes studied (mixed cellulose ester membrane, polyvinylidene fluoride membrane, and nylon membrane), the concentration efficiency using mixed cellulose ester membrane with a 0.1-μm pore size was the highest, reaching 92.62 ± 5.17%. This method was used to concentrate hepatitis A virus in water samples from Donghu Lake. Using SYBR Green real-time reverse transcription polymerase chain reaction analysis, the detection sensitivity of this method reached 101 copies/μL and its concentration efficiency reached 79.45 ± 9.88%.

Keywords

concentration filtration membrane hepatitis A virus mixed cellulose ester (MCE) membrane water samples 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • Yuting Qiao
    • 1
    • 3
  • Zhiwei Sui
    • 2
  • Guoliang Hu
    • 3
  • Huabin Cao
    • 3
  • Guoxiang Yang
    • 4
  • Yong Li
    • 4
  • Yongsong Lei
    • 4
  • Lihua Zhao
    • 1
  • Quanjiao Chen
    • 1
  1. 1.CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.National Institute of MetrologyBeijingChina
  3. 3.College of Animal Science and Technology, Institute of Animal Population HealthJiangxi Agricultural UniversityNanchangChina
  4. 4.Hubei Wildlife Epidemic Focus and Disease Surveillance CenterWuhanChina

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