Abstract
This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%、 97.3%、 97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.
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Foundation items: This work was supported by project from MOA [2006-G57(3)B-Z1] and Project from Yunnan Province (2008LA019).
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Zhao, Wh., Yang, Sb., Han, Jq. et al. Confirmed diagnosis by RT-PCR and phylogenetic analysis of peste des petits ruminants viruses in Tibet, China. Virol. Sin. 24, 573–578 (2009). https://doi.org/10.1007/s12250-009-3064-x
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DOI: https://doi.org/10.1007/s12250-009-3064-x