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Virologica Sinica

, 24:573 | Cite as

Confirmed diagnosis by RT-PCR and phylogenetic analysis of peste des petits ruminants viruses in Tibet, China

  • Wen-hua Zhao
  • Shi-biao YangEmail author
  • Jian-qiang Han
  • Mei Jiang
  • Hua-chun Li
  • Nian-zu Zhang
  • Qi-han Li
Article

Abstract

This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%、 97.3%、 97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.

Key words

Peste des petitis ruminants virus (PPRV) Tibet RT-PCR Open reading frame (ORF) Phylogenetic analysis 

CLC number

R373 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Wen-hua Zhao
    • 1
    • 3
  • Shi-biao Yang
    • 1
    Email author
  • Jian-qiang Han
    • 2
  • Mei Jiang
    • 1
  • Hua-chun Li
    • 1
  • Nian-zu Zhang
    • 1
  • Qi-han Li
    • 3
  1. 1.Laboratory of Tropical and Subtropical Animal Virology of MOANational Exotic Animal Disease Diagnostic LaboratoryKunmingChina
  2. 2.Yu Xi Agriculture Vocational Technical CollegeYu XiChina
  3. 3.Peking Union Medical CollegeTsinghua UniversityKunmingChina

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