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Relationship Between Tuber Storage Proteins and Tuber Powdery Scab Resistance in Potato

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Abstract

The potato genotypes (Solanum tuberosum L.) with russet tuber skin are generally resistant to powdery scab (Spongospora subterranea f.sp. subterranea or Sss). Lipoxygenase (LOX; EC 1.13.11.12) and patatin are two key storage proteins that are known to offer resistance to several diseases and insects. The objective of this study was to find out the relationship of these proteins in stored tubers with potato tuber powdery scab resistance, especially in russet skinned potatoes. An evaluation of potato germplasm with different tuber characteristics in a greenhouse environment over several years (2006–’11) suggests that russet skinned tuber genotypes (Mesa Russet, Centennial Russet and Russet Nugget) with negligible tuber disease severity index (DSI) and 100 % marketability were resistant to powdery scab. Higher physiological levels of LOX protein (on a dry weight basis) were negatively correlated with tuber DSI and positively correlated with tuber russet skin. Tuber total protein and patatin-lipase levels did not have a significant relationship with tuber powdery scab resistance. The proposed role of LOX protein in suberin- and/or non-suberin-mediated mechanisms of powdery scab resistance in russet skinned tubers are discussed here. The physiological levels of LOX protein can be considered as a useful marker for powdery scab resistance in potato breeding programs.

Resumen

Los genotipos de papa (Solanum tuberosum L.) con piel rugosa, generalmente son tolerantes a la enfermedad de la roña polvorienta (Spongospora subterránea f. sp. Subterránea o Sss). Lipoxigenasa (LOX; EC 1.13.11.12) y patatina son dos proteínas clave de almacenamiento que se sabe que confieren resistencia a varias enfermedades e insectos. El objetivo de este estudio fue investigar la relación de estas proteínas en tubérculos almacenados con tolerancia a la roña polvorienta, especialmente en papas de piel rugosa. Una evaluación de germoplasma de papa con diferentes características de tubérculo en un ambiente de invernadero durante varios años (2006–2011) sugiere que los genotipos con tubérculos de piel rugosa (Mesa Russet, Centennial Russet y Russet Nugget) con un índice imperceptible de severidad de la enfermedad en el tubérculo (DSI) y 100 % comercializables, fueron tolerantes a la roña polvorienta. Niveles fisiológicos más altos de la proteína LOX (en base a peso seco) estuvieron negativamente correlacionados con el DSI del tubérculo y positivamente con la piel rugosa. La proteína total del tubérculo y los niveles de patatina-lipasa no tuvieron una relación significativa con la tolerancia a la roña polvorienta. Aquí se discute el papel propuesto de la proteína LOX en mecanismos mediados y no mediados de suberina en la tolerancia a la roña polvorienta en tubérculos de piel rugosa. Los niveles fisiológicos de la proteína LOX pudieran considerarse como un marcador útil para la resistencia a la roña polvorienta en los programas de mejoramiento de papa.

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Acknowledgements

We are thankful to Andrew Houser, Mary LaRue Snell, and Teresa V. Rivera for their help during the study.

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  1. San Luis Valley Research Center, Department of Horticulture and Landscape Architecture, Colorado State University, 0249 East County Road, 9N, Center, CO, 81125, USA

    Venu Perla, Sastry S. Jayanty, David G. Holm & Robert D. Davidson

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Correspondence to Sastry S. Jayanty.

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Table S1

Evaluation of selective potato germplasm for powdery scab root gall rating in a greenhouse environment during 2006–2011. (DOCX 16 kb)

Table S2

Kendall’s tau correlation between powdery scab root gall rating, tuber DSI, unmarketable tubers, total protein, LOX and patatin-lipase levels of tubers. (DOCX 15 kb)

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Perla, V., Jayanty, S.S., Holm, D.G. et al. Relationship Between Tuber Storage Proteins and Tuber Powdery Scab Resistance in Potato. Am. J. Potato Res. 91, 233–245 (2014). https://doi.org/10.1007/s12230-013-9343-y

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