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A Multiplex PCR Method of Detecting Markers Linked to Genes Conferring Resistance to Globodera rostochiensis

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Abstract

A simple multiplex PCR method was developed for detection of the alleles H1 and Gro1-4 conferring resistance of potato to Globodera rostochiensis. SCAR markers TG689 and Gro1-4 linked to H1 and Gro1-4 genes respectively were amplified in the one PCR reaction. This allows to identify clones with resistance to G. rostochiensis originating from a single source or both of them. Effectiveness of this procedure for marker-assisted selection (MAS) was confirmed by 60 potato cultivars tested.

Resumen

Se desarrolló un método simple multiplex de PCR para la detección de los alelos H1 y Gro1-4 que confieren resistencia de la papa a Globodera rostochiensis. Se amplificaron los marcadores SCAR TG689 y Gro1-4 ligados a los genes H1 y Gro1-4 respectivamente en una reacción de PCR. Esto permite la identificación de clones con resistencia a G. rostochiensis originaria de una o ambas fuentes. Se confirmó la efectividad de este procedimiento para selección asistida con marcadores (MAS) probándolo en 60 variedades de papa.

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References

  • Barone, A., E. Ritter, U. Schachtschabel, T. Debener, F. Salamini, and C. Gebhardt. 1990. Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Molecular and General Genetics 224: 177–182.

    Article  PubMed  CAS  Google Scholar 

  • Brown, C.R., T.S. Kim, Z. Ganga, K. Haynes, D. De Jong, M. Jahn, I. Paran, and W. De Jong. 2006. Segregation of total carotenoid in high level potato germplasm and its relationship to beta-carotene hydroxylase polymorphism. American Journal of Potato Research 83: 365–372.

    Article  CAS  Google Scholar 

  • Douches, D.S., J. Coombs, K. Felcher, W.W. Kirk, C. Long, and G. Bird. 2010. Missaukee: a round white potato variety combining chip-processing with resistance to late blight, Verticillium Wilt and Golden Cyst Nematode. American Journal of Potato Research 87: 10–18.

    Article  Google Scholar 

  • Edwards, M.C., and R.A. Gibbs. 1994. Multiplex PCR: advantages, development, and applications. PCR Methods and Applications 3: 65–75.

    Google Scholar 

  • Evans, K., and D.L. Trudgill. 1992. Pest aspects of potato production. Part 1. The nematode pests of potato. In The potato crop, 2nd ed, ed. P. Harris, 438–475. London: Chapman and Hall.

    Chapter  Google Scholar 

  • Gebhardt, C., D. Mugniery, E. Ritter, F. Salamini, and E. Bonnel. 1993. Identification of RFLP markers closely linked to the H1 gene conferring resistance to Globodera rostochiensis in potato. Theoretical and Applied Genetics 85: 541–544.

    Article  CAS  Google Scholar 

  • Gebhardt, C., D. Bellin, H. Henselewski, W. Lehmann, J. Schwarzfischer, and J.P.T. Valkonen. 2006. Marker-assisted combination of major genes for pathogen resistance in potato. Theoretical and Applied Genetics 112: 1458–1464.

    Article  PubMed  CAS  Google Scholar 

  • Milczarek, D., B. Flis, and A. Przetakiewicz. 2011. Suitability of molecular markers for selection of potatoes resistant to Globodera spp. American Journal of Potato Research 88: 245–255.

    Article  CAS  Google Scholar 

  • Smith IM, McNamara DG, Scott PR, Harris KM (1992) Quarantine pests for Europe. C.A.B. Int. &EPPO

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Acknowledgments

This study was supported by the Polish Ministry of Agricultural and Rural Development within the Project for Basic Research on Biological Progress in Plant Production for 2009–2010 (decisions no. HOR hn 4040 dec - 1/09 and HOR hn 078 dec - 17/10).

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Authors and Affiliations

  1. Plant Breeding and Acclimatization Institute - National Research Institute, Research Centre Młochów, Platanowa 19, 05-831, Młochów, Poland

    Dorota Milczarek

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  1. Dorota Milczarek
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Correspondence to Dorota Milczarek.

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Milczarek, D. A Multiplex PCR Method of Detecting Markers Linked to Genes Conferring Resistance to Globodera rostochiensis . Am. J. Pot Res 89, 169–171 (2012). https://doi.org/10.1007/s12230-011-9227-y

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