Abstract
The potato variety ‘Spunta’ was previously transformed to constitutively express the cry1Ia1 gene from Bacillus thuringiensis from which three transgenic lines (SpuntaG2, SpuntaG3 and Spunta6a3) were chosen to evaluate for resistance to potato tuberworm (Phthorimaea operculella Zeller). Because potato tuberworm is becoming a serious pest in the Pacific Northwest of the United States, ‘SpuntaG2’, ‘SpuntaG3’ and ‘Spunta6a3’ were evaluated in Washington State through laboratory and field experiments. In the laboratory, both choice and no-choice experiments demonstrated that the transgenic ‘Spunta’ lines were completely resistant (100% mortality) to potato tuberworm. Potato tuberworm resistance was further supported by choice, field-cage studies in which the transgenic ‘Spunta’ lines harbored no potato tuberworm larvae at any sampling date while the controls were heavily infested (averaging 6.4 to as many 17.0 larvae per stem). These results indicate that the cry1Ia1 gene could be an effective component of potato tuberworm management in the Pacific Northwest as well as the international venues where it has already been proven effective.
Resumen
Se transformó previamente a la variedad de papa “Spunta” para que expresara constitutivamente el gen cry1Ia1 de Bacillus thuringiensis, de la cual se seleccionaron tres líneas transgénicas (SpuntaG2, SpuntaG3, y Spunta6a3) para evaluarles su resistencia a la palomilla de la papa (Phthorimaea operculella Zeller). Considerando que la palomilla de la papa se está convirtiendo en una plaga seria en el Pacífico Noroeste de los Estados Unidos, “SpuntaG2”, “SpuntaG3”, y “Spunta6a3” se evaluaron en el Estado de Washington en experimentos de laboratorio y campo. En el laboratorio, ambos, experimentos seleccionados y no seleccionados, demostraron que las líneas transgénicas de Spunta fueron completamente resistentes (100% de mortalidad) a la palomilla de la papa. La resistencia fue posteriormente respaldada por estudios de selección, en jaulas de campo, en donde las líneas transgénicas de Spunta no mantuvieron a las larvas de la palomilla en ninguna de las fechas de muestreo, mientras que los testigos estuvieron fuertemente infestados (promediando de 6.4 a tanto como 17.0 larvas por tallo). Estos resultados indican que el gen cry1Ia1 pudiera ser un componente efectivo en el manejo de la palomilla de la papa en el Pacífico Noroeste, así como a nivel internacional donde ya se ha probado que es efectivo.
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Douches, D.S., Coombs, J.J., Lacey, L.A. et al. Evaluations of Transgenic Potatoes for Resistance to Potato Tuberworm in the Laboratory and Field. Am. J. Pot Res 88, 91–95 (2011). https://doi.org/10.1007/s12230-010-9167-y
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DOI: https://doi.org/10.1007/s12230-010-9167-y