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Patterns of Genetic Diversity in Platanthera bifolia (Orchidaceae) with Respect to Life History Traits and Recent Range Expansion

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Abstract

For evolutionary and ecological analyses, genetic diversity at different scales needs to be studied in terms of biological properties, habitat, population size and population history. We surveyed Platanthera bifolia populations from six regions in northeastern Poland to determine the impact of the mating system and population history on genetic diversity. Based on variation at allozyme markers, genetic variation was relatively moderate (P = 22.3%, A = 1.48, H O = 0.083, F IS = −0.015) and similar to other Platanthera species. These parameters varied between populations (P = 13.3%–26.6%, A = 1.26–1.66, H O = 0.055–0.111, F IS = −0.262–0.147). The genetic diversity patterns were shaped by different proportions of facilitated selfing and/or outcrossing, resulting in positive and negative F IS values, respectively. No relationship between inbreeding coefficient and population size, however, and no impact of apomixis on the level of genetic diversity of P. bifolia were found. The relatively low level of genetic differentiation among the investigated regions (F CT = 0.002, P > 0.05) and among populations (F ST = 0.048, P < 0.001), and the lack of a significant relationship between genetic and geographical distance, are discussed in the context of possible scenaria of postglacial expansion.

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References

  • Ackerman JD (1998) Evolutionary potential in orchids: patterns and strategies for conservation. Selbyana 19:8–14

    Google Scholar 

  • Adamowski W (1996) Apofityzm wybranych gatunków storczykowatych (Orchidaceae) i jego uwarunkowania ekologiczne (Apophytism of orchid species (Orchidaceae) and its ecological conditioning). PhD. Thesis, Warsaw University, Białowieża

  • Adamowski W (1998) Colonization success of orchids in disturbed habitats. In Falińska K. (ed) Plant population biology. W. Szafer Institute of Botany, PAN, Kraków, pp 165–172

    Google Scholar 

  • Barrett SCH, Husband BC (1990) The genetics of plant migration and colonization. In Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding and genetic resources. Sinauer Associates, Sunderland, pp 254–277

    Google Scholar 

  • Bowless ML (1999) Eastern prairie fringed orchid Platanthera leucophaea (Nuttall) Lindley. Recovery Plan for Region 3. U.S. Fish and Wildlife Service, Fort Snelling. Unpublished manucript. Available at: http://www.fws.gov/midwest/endangered/plants/

  • Brzosko E (2003) The dynamics of island populations of Platanthera bifolia in the Biebrza National Park (NE Poland). Ann Bot Fenn 40:243–253

    Google Scholar 

  • Brzosko E, Wróblewska A (2003a) Low allozymic variation in two island populations of Listera ovata (Orchidaceae). Ann Bot Fenn 40:309–315

    Google Scholar 

  • Brzosko E, Wróblewska A (2003b) Genetic variation and clonal diversity in island Cephalanthera rubra populations from the Biebrza National Park. Bot J Linn Soc 143:99–108

    Article  Google Scholar 

  • Brzosko E, Ratkiewicz M, Wróblewska A (2002a) Allozyme differentiation and genetic structure of Lady's slipper island populations (Cypripedium calceolus) in NE Poland. Bot J Linn Soc 138:433–440

    Article  Google Scholar 

  • Brzosko E, Wróblewska A, Ratkiewicz M (2002b) Spatial genetic structure and clonal diversity of island populations of Lady's slipper (Cypripedium calceolus) from the Biebrza National Park (northeast Poland). Molec Ecol 11:2499–2509

    Article  CAS  Google Scholar 

  • Cockerham CC (1969) Variance of gene frequency. Evolution 23:72–84

    Article  Google Scholar 

  • Cowden NFF (1993) A biosystematic study of Platanthera ciliaris, P. blephariglottis, and P. ×bicolor, emphasizing the northern portion of their range. Ph.D. Dissertation, Miami University, Oxford, Ohio

  • Dafni A (1992) Pollination ecology: a practical approach. Oxford University Press, New York

    Google Scholar 

  • Ellstrand NC, Ellam DR (1993) Population genetic consequences of small population size. Implications for plant conservation. Annual Rev Ecol Syst 24:217–242

    Article  Google Scholar 

  • Forrest AD, Hollinsworth ML, Hollinsworth PM, Sydney C, Bateman RM (2004) Population genetic structure in European populations of Spiranthes romanzoffiana set in the context of other genetic studies on orchids. Heredity 92:218–227

    Article  PubMed  CAS  Google Scholar 

  • Frankham R, Ballou JD, Briscoe DA (2003) Introduction to conservation genetics. Cambridge University Press, Cambridge

    Google Scholar 

  • Gitzendanner MA, Soltis PS (2000) Patterns of genetic variation in rare and widespread plant congeners. Amer J Bot 87:777–786

    Article  Google Scholar 

  • Goodwillie C, Kalisz S, Eckert CG (2005) The evolutionary enigma of mixed mating systems in plants: Occurrence, Theoretical Explanation, and Empirical Evidence. Annual Rev Ecol Evol Syst 36:47–79

    Article  Google Scholar 

  • Goudet J (2001) PCA-GEN, ver. 1.2. Institute of Ecology, Lausanne. Available at: http://www2.unil.ch/popgen/softwares/pcagen.htm

  • Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In Brown AHD, Clegg MT, Kahler AL, Weir BS, (eds) Plant population genetics, breeding, and genetic resource. Sinauer Associates, Sunderland, pp 43–63

    Google Scholar 

  • Hamrick JL, Godt MJW (1996) Effects of live history traits on genetic diversity in plant species. Philos Trans Royal Soc Biol Sci 351:1291–1298

    Article  Google Scholar 

  • Hardy OJ, Vekemans X (2002) SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Molec Ecol Notes 2:618–620

    Article  Google Scholar 

  • Hultén E, Fries M (1986) Atlas of north European vascular plants north of the tropic of cancer. Koeltz Scientific Books, Königstein

    Google Scholar 

  • Johnson SD, Edwards TJ (2000) The structure and function of orchid pollinia. Pl Syst Evol 222:243–269

    Article  Google Scholar 

  • Kahmen S, Poschlod P (2000) Population size, plant performance, and genetic variation in the rare plant Arnica montana L. in the Rhön, Germany. Basic Appl Ecol 1:43–51

    Article  Google Scholar 

  • Legendre P, Legendre L (1998) Numerical ecology. Ed. 2. Elsevier Science BV, Amsterdam

    Google Scholar 

  • Lloyd DG, Schoen DJ (1992) Self-fertilization and cross-fertilization in plants. I. Functional dimensions. Int J Pl Sci 153:358–369

    Article  Google Scholar 

  • Loveless MD, Hamrick JL (1984) Ecological determinants of genetic structure in plant populations. Annual Rev Ecol Syst 15:65–95

    Article  Google Scholar 

  • Maad J (2002) Selection and floral evolution in Platanthera bifolia and P. chlorantha (Orchidaceae). Comprehesive Summaries of Uppsala Dissertations from the Faculty of Science and Technology. Acta Universitatis Upsaliensis, Uppsala

  • Maad J, Alexandersson R (2004) Variable selection in Platanthera bifolia (Orchidaceae): phenotypic selection differed between sex functions in a drought year. J Evol Biol 17:642–650

    Article  PubMed  CAS  Google Scholar 

  • Maad J, Reinhammar LG (2004) Incidence of geitonogamy differs between two populations in the hawkmoth-pollinated Platanthera bifolia (Orchidaceae). Canad J Bot 82:1586–1593

    Article  Google Scholar 

  • Machon N, Bardin P, Mazer SJ, Moret J, Godelle B, Austerlitz F (2003) Relationship between genetic structure and seed and pollen dispersal in the endangered orchid Spiranthes spiralis. New Phytol 157:677–687

    Article  Google Scholar 

  • Miller MP (1997) Tools for population genetic analyses (TFPGA) 1.3: A Windows program for the analysis of allozyme and molecular population genetic data. Available at: http://www.marksgeneticsoftware.net/tfpga.htm

  • Mitton JB (1989) Physiological and demographic variation associated with allozyme variation. In Soltis DE, Soltis PS (eds) Isozymes in plant biology. Dioscorides Press, Portland, pp 127–145

  • Nazarov VV, Gerlach G (1997) The potential seed productivity of orchid flowers and peculiarities of their pollination systems. Lindleyana 12:188–204

    Google Scholar 

  • Nei M (1978) Genetic distance between populations. Amer Naturalist 106:283–292

    Google Scholar 

  • Nilsson LA (1978) Pollination ecology and adaptation in Platanthera chlorantha (Orchidaceae). Bot Notiser 131:35–51

    Google Scholar 

  • Nilsson LA (1988) The evolution of flowers with deep corolla tubes. Nature 334:147–149

    Article  Google Scholar 

  • Nilsson LA (1992) Orchid pollination biology. Trends Ecol Evol 7:255–259

    Article  Google Scholar 

  • Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molec Ecol 13:1143–1155.

    Article  CAS  Google Scholar 

  • Peakall R, Beattie AJ (1991) The genetic consequences of worker ant pollination in a self-compatible, clonal orchid. Evolution 45:1837–1848

    Article  Google Scholar 

  • Raymond ML, Rousset F (1995) GENEPOP (version 1.2), a population genetics software for exact tests and ecumenism. Heredity 86:248–249

    Google Scholar 

  • Richardson BJ, Adams M, Baverstock PR (1986) Allozyme electrophoresis: a handbook for animal systematics and population studies. Academic Press, Sydney

    Google Scholar 

  • Rosenwich UL, Pettway RE, McDonald BA, Duncan RE, Frederiksen NA (1999) Genetic structure and temporal dynamics of Colletotrichum greminicola populations in a sorghum disease nursery. Phytopathology 88:1087–1093

    Article  Google Scholar 

  • Schneider S, Roessli D, Excoffier L (2000) ARLEQUIN ver. 2.000. A software for population genetics data analysis. Available at: http://www.anthro.unige.ch/arlequin

  • Slatkin M (1993) Isolation by distance in equilibrium and non-equilibrium populations. Evolution 47:264–279

    Article  Google Scholar 

  • Smith GR, Snow GE (1976) Pollination ecology of Platanthera (Habenaria) ciliaris and P. blephariglottis (Orchidaceae). Bot Gaz 137:133–140

    Article  Google Scholar 

  • Soltis DE, Soltis PS (1989) Isozymes in plant biology. Dioscorides Press, Portland

    Google Scholar 

  • Sun M, Wong KC (2001) Genetic structure of three orchid species with contrasting breeding systems using RAPD and allozyme markers. Amer J Bot 88:2180–2188

    Article  CAS  Google Scholar 

  • Tatarenko IV, Vakhrameeva MG (1998) On vegetative propagation in orchids. Byul Bot Sada I.S. Kosenko 7:155–157

    Google Scholar 

  • Vekemans X, Hardy OJ (2004) New insights from fine-scale spatial genetic structure analyses in plant populations. Molec Ecol 13:921–935

    Article  CAS  Google Scholar 

  • Vogler DW, Kalisz S (2001) Sex among the flowers: the distribution of plant mating systems. Evolution 55:202–204

    PubMed  CAS  Google Scholar 

  • Wallace LE (2002) Examining the effects of fragmentation on genetic variation in Platanthera leucophaea (Orchidaceae) Inferences from allozyme and random amplified polymorphic DNA markers. Pl Spec Biol 17:37–49

    Article  Google Scholar 

  • Wallace LE (2006) Spatial genetic structure and frequency of interspecific hybridization in Platanthera aquilonis and P. dilatata (Orchidaceae) occurring in sympatry. Amer J Bot 93:1001–1009

    Article  CAS  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Article  Google Scholar 

  • Wright S (1943) Isolation by distance. Genetics 28:114–138

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We are grateful to Adam Hermaniuk and Edyta Wasilewska for assistance in the field and in the laboratory, and thankful to Teresa Grużewska and Teresa Świerubska for helping us locate Platanthera bifolia populations in the Narew Valley and Suwałki Lakeland. The study was done under project 2P04C 048 30 financed by the Polish Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland

    Emilia Brzosko, Ada Wróblewska & Izabela Tałałaj

  2. Białowieża Geobotanical Station, Faculty of Biology, Warsaw University, Sportowa 19, 17-230, Białowieża, Poland

    Wojciech Adamowski

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  1. Emilia Brzosko
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  2. Ada Wróblewska
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  4. Wojciech Adamowski
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Correspondence to Emilia Brzosko.

 

 

Appendix 1 Genetic differentiation F ST (below diagonal) and P value ascertained using 1,000 permutations (above diagonal) between pairs of populations of Platanthera bifolia in northeastern Poland.
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Brzosko, E., Wróblewska, A., Tałałaj, I. et al. Patterns of Genetic Diversity in Platanthera bifolia (Orchidaceae) with Respect to Life History Traits and Recent Range Expansion. Folia Geobot 44, 131–144 (2009). https://doi.org/10.1007/s12224-009-9033-1

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