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The first case of Planococcus glaciei found in blood, a report from the Czech Republic

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A Correction to this article was published on 09 December 2021

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Abstract

The isolation of Planococcus glaciei (designed strain CNCTC 7660) from blood of a patient with appendicitis is reported. Species P. glaciei (type strain CGMCC 1.6846 T) was for the first time identified as an environmental bacterium acquired from a glacier in China in 2009. To reveal the identity of the isolate CNCTC 7660, the 16S rDNA sequencing and the whole genome sequencing (Illumina MiSeq, Oxford Nanopore) were performed. The level of 16S rDNA gene sequencing similarity between CNCTC 7660 and CGMCC 1.6846 T was 99.55%. Phylogenetic analysis and average nucleotide analysis (ANI) based on the whole genome sequencing confirmed that the isolate CNCTC 7660 and CGMCC1.6846 T had ANI value above the taxonomic threshold for belonging to the same species (95%). The G + C content of CNCTC 7660 DNA was 46.8% (mol/mol). Except for the growth temperature, strains CGMCC1.6846 T and CNCTC 7660 were distinguished also biochemically. Due to the lack of information about the pathogenicity of P. glaciei, the possibility that it exerts pathogenicity in persons is suggested. But for understanding the nature of this species, further cases are needed.

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Availability of data and material

The strain CNCTC 7660 is available in the Czech National Collection of Type Cultures under standard conditions. Complete genome of the strain was submitted to GenBank under accession numbers CP051177-CP051179.

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Acknowledgements

We are indebted to Eliška Křížova, Iveta Vrbová, and Eva Staňková for the technical expertise. Computational resources were supplied by the project “e-Infrastruktura CZ” (e-INFRA LM2018140) provided within the program Projects of Large Research, Development and Innovations Infrastructures.

Funding

This project was supported by MH CZ-DRO (“The National Institute of Public Health –NIPH, 75010330”).

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Authors and Affiliations

  1. National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic

    Lucia Mališová, Vladislav Jakubů & Helena Žemličková

  2. Department of Microbiology, 3Rd Faculty of Medicine, Charles University, University Hospital Kralovske Vinohrady and National Institute of Public Health, Prague, Czech Republic

    Lucia Mališová, Vladislav Jakubů & Helena Žemličková

  3. Czech National Collection of Type Cultures, National Institute of Public Health, Prague, Czech Republic

    Petra Španělová & Renáta Šafránková

  4. Department of Experimental Biology, Faculty of Science, Czech Collection of Microorganisms, Masaryk University, Brno, Czech Republic

    Ivo Sedláček

  5. Military Health Institute, Military Medical Agency, Prague, Czech Republic

    Petr Pajer

  6. National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic

    Martin Musílek

  7. Department of Clinical Microbiology, Karlovy Vary Regional Hospital, Hradec Kralove, Czech Republic

    Blanka Puchálková

  8. Department of Clinical Microbiology, Faculty of Medicine and University Hospital, Charles University, Hradec Kralove, Czech Republic

    Vladislav Jakubů & Helena Žemličková

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  1. Lucia Mališová
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  2. Petra Španělová
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Correspondence to Petra Španělová.

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Mališová, L., Španělová, P., Sedláček, I. et al. The first case of Planococcus glaciei found in blood, a report from the Czech Republic. Folia Microbiol 67, 121–127 (2022). https://doi.org/10.1007/s12223-021-00919-4

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