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Epidemiological analysis of Trueperella abortisuis isolated from cases of pig abortion of a single farm

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Abstract

The present study was designed to characterize six Trueperella (T.) abortisuis strains, cultured over a period of 5 months from fetus and abortion material of six pigs of a single farm in Mecklenburg-West Pomerania federal state, Germany. It was of interest to investigate the epidemiological relationships of the six strains among each other and whether a single bacterial clone was responsible for the abortion situation of the single farm. All six strains were identified phenotypically, by MALDI-TOF MS analysis and by phylogenetic analysis based on 16S rRNA gene and gap (encoding the glyceraldehyde 3-phosphate dehydrogenase) and tuf (encoding elongation factor tu) gene sequencing. Further genotypic comparison was performed using different genomic DNA fingerprint methods including BOX-PCR, (GTG)5-PCR, and three RAPD-PCRs. The sequence analysis of the genes gap and tuf and the genomic DNA fingerprinting results revealed, as noval findings, that the six T. abortisuis strains cultured from a single farm represent six different bacterial clones showing a genetic variability of this bacterial species in the pig population. All six T. abortisuis strains were isolated in mixed culture with several other bacterial species. However, the T. abortisuis strain, generally found in high numbers, seemed to be responsible for the abortion situation in the farm.

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References

  • Azuma R, Murakami S, Ogawa A, Okada Y, Miyazaki S, Makino T (2009) Arcanobacterium abortisuis sp. nov., isolated from a placenta of a sow following an abortion. Int J Syst Evol Microbiol 59:1469–1473

    Article  CAS  Google Scholar 

  • Bizzini A, Jaton K, Romo D, Bille J, Prod’hom G, Greub G (2011) Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains. J Clin Microbiol 49:693–696

    Article  CAS  Google Scholar 

  • Bussalleu E, Althouse GC (2017) Identification of Trueperella abortisuis contamination in extended boar semen. J Swine Health Prod 25:299–302

    Google Scholar 

  • Ghebremedhin B, Layer F, König W, König B (2008) Genetic classification and distinguishing of Staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences. J Clin Microbiol 46:1019–1025

    Article  CAS  Google Scholar 

  • Glaeser SP, Galatis H, Martin K, Kämpfer P (2013) Niabella hirudinis and Niabella drilacis sp. nov., isolated from the medicinal leech Hirudo verbana. Int J Syst Evol Microbiol 63:3487–3493

    Article  CAS  Google Scholar 

  • Hijazin M, Alber J, Lämmler C, Weitzel T, Hassan AA, Timke M, Kostrzewa M, Prenger-Berninghoff E, Zschöck M (2012a) Identification of Trueperella (Arcanobacterium) bernardiae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and by species-specific PCR. J Med Microbiol 61:457–459

    Article  CAS  Google Scholar 

  • Hijazin M, Hassan AA, Alber J, Lämmler C, Timke M, Kostrzewa M, Prenger-Berninghoff E, Zschöck M (2012b) Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species identification of bacteria of genera Arcanobacterium and Trueperella. Vet Microbiol 157:243–245

    Article  CAS  Google Scholar 

  • Hijazin M, Metzner M, Erhard M, Nagib S, Alber J, Lämmler C, Hassan AA, Prenger-Berninghoff E, Zschöck M (2012c) First description of Trueperella (Arcanobacterium) bernardiae of animal origin. Vet Microbiol 159:515–518

    Article  CAS  Google Scholar 

  • Hijazin M, Ülbegi-Mohyla H, Alber J, Lämmler C, Hassan AA, Timke M, Kostrzewa M, Prenger-Berninghoff E, Weiss R, Zschöck M (2012d) Identification of Arcanobacterium (Trueperella) abortisuis, a novel species of veterinary importance, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Berl Münch Tierärztl Wochenschr 125:32–37

    CAS  PubMed  Google Scholar 

  • Jost BH, Billington SJ (2005) Arcanobacterium pyogenes: molecular pathogenesis of an animal opportunist. A Van Leeuw J Microb 88:87–102

    Article  Google Scholar 

  • Lämmler C, Hartwigk H (1995) Actinomyces pyogenes und Arcanobacterium haemolyticum. In: Blobel H, Schließer T (eds) Handbuch der bakteriellen Infektionen bei Tieren, 2nd edn. Gustav Fischer, Jena, pp 196–240 Band II/3

    Google Scholar 

  • Lehnen A, Busse HJ, Frölich K, Krasinska M, Kämpfer P, Speck S (2006) Arcanobacterium bialowiezense sp. nov., and Arcanobacterium bonasi sp. nov., isolated from the prepuce of European bison bulls (Bison bonasus) suffering from balanoposthitis, and emended description of the genus Arcanobacterium Collins et al. 1983. Int Syst Evol Microbiol 56:861–866

    Article  CAS  Google Scholar 

  • Li X, Xing J, Li B, Wang P, Liu J (2012) Use of tuf as a target for sequence-based identification of Gram-positive cocci of the genus Enterococcus, Streptococcus, coagulase-negative Staphylococcus and Lactococcus. Ann Clin Microbiol Antimicrob 11:31–36

    Article  CAS  Google Scholar 

  • Metzner M, Erhard M, Sammra O, Nagib S, Hijazin M, Alber J, Lämmler C, Abdulmawjood A, Prenger-Berninghoff E, Zschöck M, Hassan AA (2013) Trueperella abortisuis, an emerging pathogen isolated from pigs in Germany. Berl Münch Tierärztl Wochensch 126:423–426

    Google Scholar 

  • Murray PR (2010) Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: usefulness for taxonomy and epidemiology. Clin Microbiol Infect 16:1626–1630

    Article  CAS  Google Scholar 

  • Nagib S, Glaeser SP, Eisenberg T, Sammra O, Lämmler C, Kämpfer P, Schauerte N, Geiger C, Kaim U, Prenger-Berninghoff E, Becker A, Abdulmawjood A (2017) Fatal infection in three Grey Slender Lorises (Loris lydekkerianus nordicus) caused by clonally related Trueperella pyogenes. BMC Vet Res 13:273–281

    Article  Google Scholar 

  • Risse K, Schlez K, Eisenberg T, Geiger C, Balbutskaya A, Sammra O, Lämmler C, Abdulmawjood A (2014) Phenotypical and genotypical properties of an Arcanobacterium pluranimalium strain isolated from a juvenile giraffe (Giraffa camelopardalis reticulata). J Vet Med. https://doi.org/10.1155/2014/408724

    Article  Google Scholar 

  • Sammra O, Balbutskaya A, Hijazin M, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Prenger-Berninghoff E, Timke M, Kostrzewa M, Siebert U (2014a) Further studies on Arcanobacterium phocisimile: a novel species of genus Arcanobacterium. J Vet Med. https://doi.org/10.1155/2014/923592

    Article  Google Scholar 

  • Sammra O, Balbutskaya A, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Timke M, Kostrzewa M, Prenger-Berninghoff E (2014b) Properties of an Arcanobacterium haemolyticum strain isolated from a donkey. Berl Münch Tierärztl Wochenschr 127:56–60

    PubMed  Google Scholar 

  • Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543–551

    Article  CAS  Google Scholar 

  • Ülbegi-Mohyla H, Hassan AA, Hijazin M, Alber J, Lämmler C, Abdulmawjood A, Prenger-Berninghoff E, Weiss R, Zschöck M (2011) Characterization of Arcanobacterium abortisuis by phenotypic properties and by sequencing the 16S-23S rDNA intergenic spacer region. Vet Microbiol 148:431–433

    Article  Google Scholar 

  • Wickhorst J-P, Hassan AA, Sammra O, Huber-Schlenstedt R, Lämmler C, Prenger-Berninghoff E, Timke M, Abdulmawjood A (2016) Phenotypic and genotypic approach to characterize Arcanobacterium pluranimalium isolated from bovine milk samples. Folia Microbiol (Praha) 61:405–412

    Article  CAS  Google Scholar 

  • Wickhorst J-P, Sammra O, Hassan AA, Alssahen M, Lämmler C, Prenger-Berninghoff E, Erhard M, Metzner M, Paschertz K, Timke M, Abdulmawjood A (2017) Identification of Arcanobacterium hippocoleae by MALDI-TOF MS analysis and by various genotypical properties. Res Vet Sci 115:10–12

    Article  CAS  Google Scholar 

  • Wickhorst J-P, Hassan AA, Sheet OH, Eisenberg T, Sammra O, Alssahen M, Lämmler C, Prenger-Berninghoff E, Zschöck M, Timke M, Abdulmawjood A (2018) Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus). Folia Microbiol (Praha) 63:17–22

    Article  CAS  Google Scholar 

  • Wickhorst J-P, Hassan AA, Sammra O, Alssahen M, Lämmler C, Prenger-Berninghoff E, Naggert M, Timke M, Rau J, Abdulmawjood A (2019) First report on the isolation of Trueperella abortisuis from companion animals. Res Vet Sci 125:465–467

    Article  Google Scholar 

  • Yassin AF, Hupfer H, Siering C, Schumann P (2011) Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al., 1982 emend Lehnen et al., 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium. Int J Syst Evol Microbiol 61:1265–1274

    Article  CAS  Google Scholar 

  • Ziemke F, Brettar I, Höfle M (1997) Stability and diversity of the genetic structure of a Shewanella putrefaciens population in the water column of the central Baltic. Aquat Microb Ecol 13:63–74

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurter Straße 85-91, 35392, Giessen, Germany

    Mazen Alssahen, Jörn-Peter Wickhorst, Osama Sammra, Christoph Lämmler & Ellen Prenger-Berninghoff

  2. Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität Gießen, Frankfurter Straße 92, 35392, Giessen, Germany

    Abdulwahed Ahmed Hassan

  3. Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany

    Stefanie P. Glaeser & Peter Kämpfer

  4. Entwicklung Bioanalyse, Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359, Bremen, Germany

    Markus Timke

  5. Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bünteweg 17, 30559, Hannover, Germany

    Amir Abdulmawjood

Authors
  1. Mazen Alssahen
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  2. Abdulwahed Ahmed Hassan
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  3. Jörn-Peter Wickhorst
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  4. Osama Sammra
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  5. Christoph Lämmler
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  6. Stefanie P. Glaeser
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  7. Peter Kämpfer
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  8. Markus Timke
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  9. Ellen Prenger-Berninghoff
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  10. Amir Abdulmawjood
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Correspondence to Christoph Lämmler.

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Alssahen, M., Hassan, A.A., Wickhorst, JP. et al. Epidemiological analysis of Trueperella abortisuis isolated from cases of pig abortion of a single farm. Folia Microbiol 65, 491–496 (2020). https://doi.org/10.1007/s12223-019-00753-9

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  • DOI: https://doi.org/10.1007/s12223-019-00753-9

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