Skip to main content
SpringerLink
Log in

Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus)

  • Original Article
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S–23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor-encoding genes which revealed the presence of the genes plo encoding pyolysin and nanH and nanP encoding neuraminidases; the genes fimA, fimC, and fimE encoding the fimbrial subunits FimA, FimC, and FimE; and the gene cbpA encoding collagen-binding protein CbpA. The present data give a detailed characterization of a T. pyogenes strain isolated from a brain abscess of a roebuck. However, the route of infection of the roebuck remains unclear.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abdulmawjood A, Wickhorst J, Sammra O, Lämmler C, Foster G, Wragg PN, Prenger-Berninghoff E, Klein G (2015) Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium. Mol Cell Probes 29:468–472

    Article  CAS  PubMed  Google Scholar 

  • Abdulmawjood A, Wickhorst J, Hashim O, Sammra O, Hassan AA, Alssahen M, Lämmler C, Prenger-Berninghoff E, Klein G (2016) Application of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella pyogenes isolated from various origins. Mol Cell Probes 30:205–210

    Article  CAS  PubMed  Google Scholar 

  • Belser EH, Cohen BS, Keeler SP, Killmaster CH, Bowers JW, Miller KV (2015) Epethelial presence of Trueperella pyogenes predicts site-level presence of cranial abscess disease in white-tailed deer (Odocoileus virginianus). PLoS One 10(3):1371

    Article  Google Scholar 

  • Billington SJ, Jost BH, Cuevas WA, Bright KR, Songer JG (1997) The Arcanobacterium (Actinomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiol-activated cytolysin family. J Bacteriol 179:6100–6106

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bizzini A, Jaton K, Romo D, Bille J, Prod’hom G, Greub G (2011) Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains. J Clin Microbiol 49:693–696

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cohen BS, Belser EH, Killmaster CH, Bowers JW, Irwin BJ, Yabsley MJ, Miller KV (2015a) Epizootiology of cranial abscess disease in white-tailed deer (Odocoileus virginianus) of Georgia, USA. J Wildl Dis 51:609–618

    Article  CAS  PubMed  Google Scholar 

  • Cohen BS, Belser EH, Keeler SP, Yabsley MJ, Miller KV (2015b) Isolation and genotypic characterization of Trueperella (Arcanobacterium) pyogenes recovered from active cranial abscess infections of male white-tailed deer (Odocoileus virginianus). J Zoo Wildl Med 46:62–67

    Article  PubMed  Google Scholar 

  • Ding H, Lämmler C (1996) Purification and further characterization of a haemolysin of Actinomyces pyogenes. J Vet Med B Infect Dis Vet Public Health 43:179–188

    Article  CAS  Google Scholar 

  • Eisenberg T, Nagib S, Hijazin M, Alber J, Lämmler C, Hassan AA, Timke M, Kostrzewa M, Prenger-Berninghoff E, Schauerte N, Geiger C, Kaim U, Zschöck M (2012) Trueperella pyogenes as cause of a facial abscess in a grey slender loris (Loris lydekkerianus nordicus)—a case report. Berl Münch Tierärztl Wochenschr 125:407–410

    PubMed  Google Scholar 

  • Ertaş HB, Kiliç A, Özbey G, Muz A (2005) Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed cattle kidney and identification by PCR. Turk J Vet Anim Sci 29:455–459

    Google Scholar 

  • Hassan AA, Ülbegi-Mohyla H, Kanbar T, Alber J, Lämmler C, Abdulmawjood A, Zschöck M, Weiss R (2009) Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses. J Clin Microbiol 47:124–128

    Article  CAS  PubMed  Google Scholar 

  • Hijazin M, Ulbegi-Mohyla H, Alber J, Lämmler C, Hassan AA, Abdulmawjood A, Prenger-Berninghoff E, Weiss R, Zschöck M (2011) Molecular identification and further characterization of Arcanobacterium pyogenes isolated from bovine mastitis and from various other origins. J Dairy Sci 94:1813–1819

    Article  CAS  PubMed  Google Scholar 

  • Hijazin M, Hassan AA, Alber J, Lämmler C, Timke M, Kostrzewa M, Prenger-Berninghoff E, Zschöck M (2012a) Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species identification of bacteria of genera Arcanobacterium and Trueperella. Vet Microbiol 157:243–245

    Article  CAS  PubMed  Google Scholar 

  • Hijazin M, Prenger-Berninghoff E, Sammra O, Alber J, Lämmler C, Kämpfer P, Glaeser SP, Busse HJ, Hassan AA, Abdulmawjood A, Zschöck M (2012b) Arcanobacterium canis sp. nov., isolated from otitis externa of a dog, and emended description of the genus Arcanobacterium Collins et al. 1983 emend. Yassin et al. 2011. Int J Syst Evol Microbiol 62:2201–2205

    Article  CAS  PubMed  Google Scholar 

  • Jost BH, Billington SJ (2005) Arcanobacterium pyogenes: molecular pathogenesis of an animal opportunist. A Van Leeuw J Microb 88:87–102

    Article  Google Scholar 

  • Lämmler C, Hartwigk H (1995) Actinomyces pyogenes und Arcanobacterium haemolyticum. In: Blobel H, Schließer T (eds) Handbuch der bakteriellen Infektionen bei Tieren, 2. edn. Gustav Fischer, Jena, pp 196–240 Band II/3

  • Murray PR (2010) Matrix-assisted laser desorption ionization time of-flight mass spectrometry: usefulness for taxonomy and epidemiology. Clin Microbiol Infec 16:1626–1630

    Article  CAS  Google Scholar 

  • Plamondon M, Martinez G, Raynal L, Touchette M, Valiquette L (2007) A fatal case of Arcanobacterium pyogenes endocarditis in a man with no identified animal contact: case report and review of the literature. Eur J Clin Microbiol Infect Dis 26:663–666

    Article  CAS  PubMed  Google Scholar 

  • Sammra O, Balbutskaya A, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Timke M, Kostrzewa M, Prenger-Berninghoff E (2014) Properties of an Arcanobacterium haemolyticum strain isolated from a donkey. Berl Münch Tierärztl Wochenschr 127:10–14

    Google Scholar 

  • Santos TMA, Caixeta LS, Machado VS, Rauf AK, Gilbert RO, Bicalho RC (2010) Antimicrobial resistance and presence of virulence factor genes in Arcanobacterium pyogenes isolated from the uterus of postpartum dairy cows. Vet Microbiol 145:84–89

    Article  CAS  PubMed  Google Scholar 

  • Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clinic Inf Dis 49:543–551

    Article  CAS  Google Scholar 

  • Silva E, Gaivão M, Leitão S, Jost BH, Carneiro C, Vilela CL, Lopes da Costa L, Mateus L (2008) Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis. Vet Microbiol 25:111–118

    Article  Google Scholar 

  • Ülbegi-Mohyla H, Hassan AA, Kanbar T, Alber J, Lämmler C, Prenger-Berninghoff E, Weiss R, Siebert U, Zschöck M (2009) Synergistic and antagonistic hemolytic activities of bacteria of genus Arcanobacterium and CAMP-like hemolysis of Arcanobacterium phocae and Arcanobacterium haemolyticum with Psychrobacter phenylpyruvicus. Res Vet Sci 87:186–188

    Article  PubMed  Google Scholar 

  • Ülbegi-Mohyla H, Hijazin M, Alber J, Lämmler C, Hassan AA, Abdulmawjood A, Prenger-Berninghoff E, Weiss R, Siebert U, Zschöck M (2010a) Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties. J Vet Sci 11:265–267

    Article  PubMed  PubMed Central  Google Scholar 

  • Ülbegi-Mohyla H, Hassan AA, Alber J, Lämmler C, Prenger-Berninghoff E, Weiss R, Zschöck M (2010b) Identification of Arcanobacterium pluranimalium isolated from a dog by phenotypic properties and by PCR mediated characterization of various molecular targets. Vet Microbiol 142:458–460

    Article  PubMed  Google Scholar 

  • Wickhorst J-P, Hassan AA, Sammra O, Huber-Schlenstedt R, Lämmler C, Prenger-Berninghoff E, Timke M, Abdulmawjood A (2016) Phenotypic and genotypic approach to characterize Arcanobacterium pluranimalium isolated from bovine milk samples. Folia Microbiol (Praha) 61:405–412

    Article  CAS  Google Scholar 

  • Yassin AF, Hupfer H, Siering C, Schumann P (2011) Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al., 1982 emend Lehnen et al., 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium. Int J Syst Evol Microbiol 61:1265–1274

    Article  CAS  PubMed  Google Scholar 

  • Zhao K, Tian Y, Yue B, Wang H, Zhang X (2013) Virulence determinants and biofilm production among Trueperella pyogenes recovered from abscesses of captive forest musk deer. Arch Microbiol 195:203–209

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392, Giessen, Germany

    Jörn-Peter Wickhorst, Osama Sammra, Mazen Alssahen & Christoph Lämmler

  2. Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq

    Abdulwahed Ahmed Hassan & Omar Hashim Sheet

  3. Landesbetrieb Hessisches Landeslabor, Schubertstraße 60, 35392, Giessen, Germany

    Tobias Eisenberg & Michael Zschöck

  4. Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurterstraße 85-91, 35392, Giessen, Germany

    Ellen Prenger-Berninghoff

  5. Entwicklung Bioanalyse, Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359, Bremen, Germany

    Markus Timke

  6. Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559, Hannover, Germany

    Amir Abdulmawjood

Authors
  1. Jörn-Peter Wickhorst
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Abdulwahed Ahmed Hassan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Omar Hashim Sheet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tobias Eisenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Osama Sammra
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mazen Alssahen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Christoph Lämmler
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ellen Prenger-Berninghoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Michael Zschöck
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Markus Timke
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Amir Abdulmawjood
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Christoph Lämmler.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wickhorst, JP., Hassan, A.A., Sheet, O.H. et al. Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus). Folia Microbiol 63, 17–22 (2018). https://doi.org/10.1007/s12223-017-0533-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12223-017-0533-8

Search

Navigation