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Diversity of actinomycetes isolated from subseafloor sediments after prolonged low-temperature storage

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Abstract

Subseafloor sediments present an untapped source of novel bacterial species with industrially important bioactivities. Subseafloor core samples collected during the Integrated Ocean Drilling Program Expeditions 315, 316, and 331 and stored in Kochi Core Center at −80 °C for 1 to 4 years were used for cultivation-based study of viable actinomycetes. In total, more than 100 actinomycete-like colonies were isolated from two deep-frozen subseafloor sediment samples. Isolated actinomycetes showed close similarity to known Actinotalea, Dietzia, Gordonia, Isoptericola, Microbacterium, Nocardia, Rhodococcus, Pseudonocardia, Streptomyces, and Tsukamurella species and were halotolerant. Bioactivity assays revealed that two of the isolates were producing potent antibacterial compound(s) and one isolate was having antifungal activity. Our study demonstrated that deep-frozen subseafloor core samples could be a potential source of viable actinomycetes, which may be used in drug discovery.

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Acknowledgments

This research used samples provided by the Integrated Ocean Drilling Program (IODP). This work was performed through Program to Disseminate Tenure Tracking System of the Ministry of Education, Culture, Sports, Science and Technology, The Japanese Government. The authors thank C. Ihara for technical assistance and Kochi Prefectural Deep Seawater Laboratory for providing deep seawater.

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Authors and Affiliations

  1. Oceanography Section, Science Research Center, Kochi University, IMT-MEXT, Kohasu, Oko-cho, Nankoku, Kochi, 783-8505, Japan

    Dana Ulanova

  2. Evaluation and Support Organization for Young Researchers, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, 783-8505, Japan

    Kian-Sim Goo

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  1. Dana Ulanova
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  2. Kian-Sim Goo
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Correspondence to Dana Ulanova.

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Ulanova, D., Goo, KS. Diversity of actinomycetes isolated from subseafloor sediments after prolonged low-temperature storage. Folia Microbiol 60, 211–216 (2015). https://doi.org/10.1007/s12223-014-0361-z

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  • DOI: https://doi.org/10.1007/s12223-014-0361-z

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