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Pathogenic analysis of Vibrio alginolyticus infection in a mouse model

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Abstract

Vibrio alginolyticus is a Gram-negative halophilic bacterium and has been recognized as an opportunistic pathogen to both humans and marine animals. So far, most studies have been focused on marine animals and few reports have been aimed at mammals, including human. In this study, we first established a mouse model to understand the pathogenic mechanism of V. alginolyticus infection. After infection via intraperitoneal injection, hematological and liver function indicators were evaluated and serum interleukin (IL)-1β and IL-6 expression were detected by ELISA. Furthermore, we compared the virulence of two V. alginolyticus strains, ATCC17749T and E0666. The results demonstrated that V. alginolyticus infection causes robust lung and liver damage and induces changes in IL-1β, IL-6, hematological, and liver indicators. In addition, the ATCC17749T strain appeared to be more virulent than the E0666 strain. Better understanding of the pathogenic mechanism of V. alginolyticus infection should guide effective prevention and therapy for V. alginolyticus infection.

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Acknowledgments

This work was supported by Major PLA Research Project of “The 12th Five-Year Plan” for Medical Science Development (no. BWS12J014), China Science Foundation Postdoctoral Fellowship Grant (no. 2012 M521929), and the National Natural Science Foundation of China (no. 81201265).

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Authors and Affiliations

  1. Department of Laboratory Medicine, General Hospital of Jinan Military Region of PLA, Jinan, 250031, Shandong Province, People’s Republic of China

    Xiao-Fei Liu, Helin Zhang, Yanwen Gong, Yingjian Chen, Yuan Cao & Chengjin Hu

  2. Department of Gastroenterology, Weifang Hospital of Traditional Chinese Medicine, Weifang, 261041, Shandong Province, People’s Republic of China

    Xingshan Liu

Authors
  1. Xiao-Fei Liu
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  2. Helin Zhang
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  3. Xingshan Liu
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  4. Yanwen Gong
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  5. Yingjian Chen
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  6. Yuan Cao
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  7. Chengjin Hu
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Correspondence to Chengjin Hu.

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Liu, XF., Zhang, H., Liu, X. et al. Pathogenic analysis of Vibrio alginolyticus infection in a mouse model. Folia Microbiol 59, 167–171 (2014). https://doi.org/10.1007/s12223-013-0279-x

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  • DOI: https://doi.org/10.1007/s12223-013-0279-x

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