Abstract
Bacteria-assisted bioremediation is widely recognized as a low-cost method to minimize the consequences of soil pollution with toxic metals originating from industrial sites. Strains used in bioremediation have to deal with high metal load via biosorption, reduction, bioprecipitation, metal sequestration, and/or chelation. Actinobacteria, and streptomycetes in particular, are considered a perspective group for bioremediation as natural soil inhabitants with extensive secondary metabolism. Nevertheless, there is no reference information on survival of the model streptomycetes in the presence of the most abundant metal pollutants. Also, there are no reports describing the selection approaches towards improvement of bioremediation properties. In this work, the resistance of Streptomyces coelicolor M145 and Streptomyces sioyaensis Lv81 to certain transition metals and their growth under different pH values are described for the first time. Spontaneous chromate-resistant S. sioyaensis Lv81-138 strain was selected in the course of this work. Strain Lv81-138 is the most efficient actinobacterial Cr(VI) reducer reported so far, capable of converting 12 mmol/L of Cr(VI) into Cr(III) in a medium supplemented with 50 mmol/L K2CrO4.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abbas AS, Edwards C (1990) Effects of metals on Streptomyces coelicolor growth and actinorhodin production. Appl Environ Microbiol 56:675–680
Abbas A, Edwards C (1989) Effects of metals on a range of Streptomyces species. Appl Environ Microbiol 55:2030–2035
Addour L, Belhocine D, Boudries N, Comeau Y, Pauss A, Mameri N (1999) Zinc uptake by Streptomyces rimosus biomass using a packed-bed column. J Chem Technol Biotechnol 74:1089–1095
Bentley SD, Chater KF, Cerdeno-Terraga A-M et al (2002) Complete genome sequence of the model actinomycete Streptomyces coelicolor A(3)2. Nature 417:141–147
Brofft JE, McArthur JV, Shimkets LJ (2002) Recovery of novel bacterial diversity from a forested wetland impacted by reject coal. Environ Microbiol 4:764–769
Dávila Costa JS, Kothe E, Abate CM, Amoroso MJ (2012) Unraveling the Amycolatopsis tucumanensis copper-resistome. Biometals 25:905–917
Fernandez PM, Figueroa LIC, Farina JI (2010) Critical influence of culture medium and Cr(III) quantification protocols on the interpretation of Cr(VI) bioremediation by environmental fungal isolates. Water Air Soil Pollut 206:283–293
Haferburg G, Kothe E (2010) Metallomics: lessons for metalliferous soil remediation. Appl Microbiol Biotechnol 87:1271–1280
Hesketh A, Kock H, Mootien S, Bibb M (2009) The role of absC, a novel regulatory gene for secondary metabolism, in zinc-dependent antibiotic production in Streptomyces coelicolor A3(2). Mol Microbiol 74:1427–1444
Hodgson DA (2000) Primary metabolism and its control in streptomycetes: a most unusual group of bacteria. Adv Microb Physiol 42:47–238
Javaid M, Sultan S (2012) Plant growth promotion traits and Cr (VI) reduction potentials of Cr (VI) resistant Streptomyces strains. J Bas Microbiol. doi:10.1002/jobm.201200032
Johnson DB, Hallberg KB (2009) Carbon, iron and sulfur metabolism in acidophilic microorganisms. Adv Microbiol Physiol 54:201–255
Johnson DB, Macvicar JHM, Rolfe S (1987) A new solid medium for the isolation and enumeration of Thiobacillus ferrooxidans and acidophilic heterotrophic bacteria. J Microbiol Meth 7:9–18
Kontro M, Lignell U, Hirvonen MR, Nevalainen A (2005) pH effects on 10 Streptomyces spp. growth and sporulation depend on nutrients. Lett Appl Microbiol 41:32–38
Kothe E, Dimkpa C, Haferburg G, Schmidt A, Schütze E (2010) Streptomycete heavy metal resistance: extracellular and intracellular mechanisms. Soil Biology. Soil Heavy Met 19:225–235
Mabrouk Mona EM (2008) Statistical optimization of medium components for chromate reduction by halophilic Streptomyces sp. MS-2. Afr J Microbiol Res 2:103–109
Margesin R, Płaza GA, Kasenbacher S (2011) Characterization of bacterial communities at heavy-metal-contaminated sites. Chemosphere 82:1583–1588
Morales DK, Ocampo W, Zambrano MM (2007) Efficient removal of hexavalent chromium by a tolerant Streptomyces sp. affected by the toxic effect of metal exposure. J Appl Microbiol 103:2704–2712
Myronovskyy M, Ostash B, Ostash I, Fedorenko V (2009) A gene cloning system for the siomycin producer Streptomyces sioyaensis NRRL-B5408. Folia Microbiol 54:91–96
Polti MA, Amoroso MJ, Abate CM (2010) Chromate reductase activity in Streptomyces sp. MC1. J Gen Appl Microbiol 56:11–18
Sagar S, Dwivedi A, Yadav S, Tripathi M, Kaistha SD (2012) Hexavalent chromium reduction and plant growth promotion by Staphylococcus arlettae strain Cr11. Chemosphere 86:847–852
Sahmoune MN, Louhab K, Adda J (2008) Chromium biosorption by waste biomass of Streptomyces rimosus generated from the antibiotic industry. J Appl Sci Res 4:1076–1082
Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340
Summers AO (2009) Damage control: regulating defenses against toxic metals and metalloids. Curr Opin Microbiol 12:138–144
Voelker F, Altaba S (2001) Nitrogen source governs the patterns of growth and pristinamycin production in ‘Streptomyces pristinaespiralis’. Microbiology 147:2447–2459
Zakaliukina IV, Zenova GM, Zviagintsev DG (2004) Growth and morphological differentiation of acidophilic and neutrophilic soil Streptomyces. Mikrobiologiia 73:89–93
Acknowledgments
The work was supported by grant Bg-97P from the Ministry of Education and Science of Ukraine (to VF) and DAAD fellowships to TG, BO, and YH.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
ESM 1
(PDF 416 kb)
Rights and permissions
About this article
Cite this article
Gren, T., Ostash, B., Hrubskyy, Y. et al. Influence of transition metals on Streptomyces coelicolor and S. sioyaensis and generation of chromate-reducing mutants. Folia Microbiol 59, 147–153 (2014). https://doi.org/10.1007/s12223-013-0277-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12223-013-0277-z