Skip to main content
SpringerLink
Log in

The effect of maize silage as co-substrate for swine manure on the bacterial community structure in biogas plants

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The qualitative and quantitative changes in the bacterial community composition in two mesophilic, commercially used biogas plants were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR. The main objective was to evaluate the influence of the co-substrate maize silage on total bacteria and some selected bacterial groups by comparing full-scale reactors fed solely with pig manure or additionally with maize silage. DGGE fingerprints reflected shifts in the bacterial community structure associated with maize silage as co-substrate and the real-time PCR results showed clear changes in the quantitative composition of the bacterial consortia of each fermenter. A clear dominance of Clostridia in all surveyed fermenters and considerably lower abundance of Bacteroidetes in the biogas plant fed with maize silage was shown.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bartosch S, Fite A, Macfarlane GT, McMurdo MET (2004) Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota. Appl Environ Microbiol 70:3575–3581. doi:10.1128/AEM.70.6.3575-3581.2004

    Article  PubMed  CAS  Google Scholar 

  • Cook KL, Rothrock MJ Jr, Lovanh N, Sorrell JK, Loughrin JH (2010) Spatial and temporal changes in the microbial community in an anaerobic swine waste treatment lagoon. Anaerobe 16:74–82. doi:10.1016/j.anaerobe.2009.06.003

    Article  PubMed  CAS  Google Scholar 

  • Fischer SG, Lerman LS (1983) DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc Natl Acad Sci USA 80:1579–1583

    Article  PubMed  CAS  Google Scholar 

  • Gardes M, Bruns T (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizas and rusts. Mol Ecol 2:113–118. doi:10.1111/j.1365-294X.1993.tb00005.x

    Article  PubMed  CAS  Google Scholar 

  • Goberna M, Gadermaier M, García C, Wett B, Insam H (2010a) Adaptation of methanogenic communities to the cofermentation of cattle excreta and olive mill wastes at 37°C and 55°C. Appl Environ Microbiol 76:6564–6571. doi:10.1128/AEM.00961-10

    Article  PubMed  CAS  Google Scholar 

  • Goberna M, Franke-Whittle IH, Schoen M, Insam H (2010b) Mesophilic and thermophilic co-fermentation of cattle manure and olive mill wastes in pilot anaerobic digesters. Biomass Bioenerg 34:340–346. doi:10.1016/j.biombioe.2009.11.005

    Article  CAS  Google Scholar 

  • Kaparaju P, Luostarinen S, Kalmari E, Rintala J (2002) Co-digestion of energy crops and industrial confectionery by-products with cow manure: batch scale and farm-scale evaluation. Water Sci Technol 45:275–280

    PubMed  CAS  Google Scholar 

  • Knapp BA, Seeber J, Rief A, Meyer E, Insam H (2010) Bacterial community composition of the gut microbiota of Cylindroiulus fulviceps (Diplopoda) as revealed by molecular fingerprinting and cloning. Folia Microbiol 55:489–496. doi:10.1007/s12223-010-0081-y

    Article  CAS  Google Scholar 

  • Krause L, Diaz NN, Edwards RA et al (2008) Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. J Biotechnol 136:91–101. doi:10.1016/j.biotec.2008.06.003

    Article  PubMed  CAS  Google Scholar 

  • Kröber M, Bekel T, Diaz NN et al (2009) Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing. J Biotechnol 142:38–49. doi:10.1016/j.biotec.2009.02.010

    Article  PubMed  Google Scholar 

  • Kuypers MMM, Sliekers AO, Lavik G, Schmid M, Jørgensen BB, Kuenen JG et al (2003) Anaerobic ammonium oxidation by bacteria in the Black Sea. Nature 422:608–611

    Article  PubMed  CAS  Google Scholar 

  • Lethomäki A, Huttunen S, Rintala JA (2007) Laboratory investigations on co-digestion of energy crops and crop residues with cow manure ratio. Resour Conserv Recy 51:591–609. doi:10.1016/j.resconrec.2006.11.004

    Article  Google Scholar 

  • Liu FH, Wang SB, Zhang JS, Zhang J, Yan X, Zhou HK, Zhao GP, Zhou ZH (2009) The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis. J Appl Microbiol 106:952–966. doi:10.1111/j.1365-2672.2008.04064.x

    Article  PubMed  CAS  Google Scholar 

  • Mrázek J, Štrosová L, Fliegerová K, Kott T, Kopečný J (2008) Diversity of insect intestinal microflora. Folia Microbiol 53:229–233. doi:10.1007/s12223-008-0032-z

    Article  Google Scholar 

  • Munk B, Bauer C, Gronauer A, Lebuhn M (2010) Population dynamics of methanogens during acidification of biogas fermenters fed with maize silage. Eng Life Sci 6:496–508. doi:10.1002/elsc.201000056

    Article  Google Scholar 

  • Muyzer G, Dewaal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700

    PubMed  CAS  Google Scholar 

  • Nettman E, Bergmann I, Mundt K, Linke B, Klocke M (2008) Archaea diversity within a commercial biogas plant utilizing herbal baiomass determined by 16S rDNA and mcrA analysis. J Appl Microbiol 105:1835–1850. doi:10.1111/j.1365-2672.2008.03949.x

    Article  Google Scholar 

  • Nettman E, Bergmann I, Pramschüfer S, Mundt K, Plogsties V, Herrmann C, Klocke M (2010) Polyphasic analysis of methanogenic archaeal communities in agricultural biogas plants. Appl Environ Microbiol 76:2540–2548. doi:10.1128/AEM.01423-09

    Article  Google Scholar 

  • Peu P, Brugere H, Pourcher AM, Kerouredan M, Godon JJ, Delgenes JP, Dabert P (2006) Dynamics of a pig slurry microbial community during anaerobic storage and management. Appl Environ Microbiol 72:3578–3585. doi:10.1128/AEM.72.5.3578-3585.2006

    Article  PubMed  CAS  Google Scholar 

  • Salzman NH, Hung K, Haribhai D, Chu H, Karlsson-Sjberg J, Amir E, Teggatz P, Barman M, Hayward M, Eastwood D, Stoel M, Zhou Y, Sodergren E, Weinstock GM, Bevins CL, Williams CB, Bos NA (2009) Enteric defensins are essential regulators of intestinal microbial ecology. Nature Immunol 11:76–82

    Article  Google Scholar 

  • Schlüter A, Bekel T, Diaz NN et al (2008) The methagenome of biogas-producing microbial community of a production-scale biogas plant fermener analyzed by the 454-pyrosequencing technology. J Biotechnol 136:77–90. doi:10.1016/j.biotec.2008.05.008

    Article  PubMed  Google Scholar 

  • Snell-Castro R, Godon JJ, Delgenes JP, Dabert P (2005) Characterization of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis. FEMS Microbiol Ecol 52:229–242. doi:10.1016/j.femsec.2004.11.016

    Article  PubMed  CAS  Google Scholar 

  • Somayaji D, Khanna S (1994) Biomethanation of rice and wheat straw. World J Microbiol Biotechnol 10:521–523

    Article  Google Scholar 

  • Song M, Shin S, Hwang S (2010) Methanogenic population dynamics assessed by real-time quantitative PCR in sludge granule in upflow anaerobic sludge blanket treating swine wastewater. Bioresource Technol 101:S23–S28. doi:10.1016/j.biortech.2009.03.054

    Article  CAS  Google Scholar 

  • Wang H, Lehtomäki A, Tolvanen K, Puhakka J, Rintala J (2009) Impact of crops species on bacterial community structure during anaerobic co-digestion of crops and cow manure. Bioresource Technol 100:2311–2315. doi:10.1016/j.biortech.2008.10.040

    Article  CAS  Google Scholar 

  • Weiland P (2003) Production and energetic use of biogas from energy crops and wastes in Germany. Appl Biochem Biotechnol 109:263–274. doi:10.1385/ABAB:109:1-3:263

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Agency for Agricultural Research of the Ministry of Agriculture of Czech Republic (project no. QI92A286) and Czech Science Foundation (project no. P503/10/P394).

Author information

Authors and Affiliations

  1. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic

    K. Fliegerová & J. Mrázek

  2. ENKI, o.p.s., Dukelská 1, Třeboň, Czech Republic

    M. Kajan

  3. Institute of Microbiology, University of Innsbruck, Technikerstr. 25d, 6020, Innsbruck, Austria

    S. M. Podmirseg & H. Insam

Authors
  1. K. Fliegerová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Mrázek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Kajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. M. Podmirseg
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Insam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Fliegerová.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fliegerová, K., Mrázek, J., Kajan, M. et al. The effect of maize silage as co-substrate for swine manure on the bacterial community structure in biogas plants. Folia Microbiol 57, 281–284 (2012). https://doi.org/10.1007/s12223-012-0125-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12223-012-0125-6

Keywords

Search

Navigation