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Significant reduction in toxicity, BOD, and COD of textile dyes and textile industry effluent by a novel bacterium Pseudomonas sp. LBC1

Folia Microbiologica volume 57, pages 115–122 (2012)Cite this article

Abstract

The 16S rRNA sequence analysis and biochemical characteristics were confirmed that the isolated bacterium is Pseudomonas sp. LBC1. The commonly used textile dye, Direct Brown MR has been used to study the fate of biodegradation. Pseudomonas sp. LBC1 showed 90% decolorization of Direct Brown MR (100 mg/L) and textile industry effluent with significant reduction in COD and BOD. The optimum condition for decolorization was 7.0 pH and 40°C. Significant increase in a activity of extracellular laccase suggested their possible involvement in decolorization of Direct Brown MR. Biodegradation metabolites viz. 3,6-dihydroxy benzoic acid, 2-hydroxy-7-aminonaphthol-3-sulfonic acid, and p-dihydroperoxybenzene were identified on the basis of mass spectra and using the 1.10 beta Shimadzu NIST GC–MS library. The Direct Brown MR and textile industry effluent were toxic to Sorghum bicolor and Vigna radiata plants as compared to metabolites obtained after decolorization. The Pseudomonas sp. LBC1 could be useful strain for decolorization and detoxification of textile dyes as well as textile industry effluent.

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Acknowledgement

One of the authors (Dr. Amar A. Telke) is thankful to BK21 program of South Korea for financial assistance.

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Affiliations

  1. Division of Applied Life Sciences (BK21), PMBBRC, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam, 660-701, South Korea

    Amar A. Telke & Seon-Won Kim

  2. Department of Biochemistry, Shivaji University, Kolhapur, 416004, India

    Sanjay P. Govindwar

Authors
  1. Amar A. Telke
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  2. Seon-Won Kim
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  3. Sanjay P. Govindwar
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Correspondence to Amar A. Telke.

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Telke, A.A., Kim, SW. & Govindwar, S.P. Significant reduction in toxicity, BOD, and COD of textile dyes and textile industry effluent by a novel bacterium Pseudomonas sp. LBC1. Folia Microbiol 57, 115–122 (2012). https://doi.org/10.1007/s12223-012-0103-z

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  • DOI: https://doi.org/10.1007/s12223-012-0103-z

Keywords

  • Chemical Oxygen Demand
  • Biochemical Oxygen Demand
  • Textile Industry
  • Nutrient Broth
  • Laccase Activity