Abstract
Genetic diversity among 43 petroleum hydrocarbon-degrading Pseudomonas belonging to four different species and the type strain Pseudomonas aeruginosa MTCC1034 was assessed by using restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR)-amplified 16S–23S rDNA intergenic spacer regions (ISRs) polymorphism. PCR amplification from all Pseudomonas species yielded almost identical ISR amplicons of “?” 800 bp and in nested PCR of “?” 550 bp. The RFLP analysis with MboI and AluI revealed considerable intraspecific variations within the Pseudomonas species. The dendrogram constructed on the basis of the PCR-RFLP patterns of 16S–23S rDNA intergenic spacer regions differentiated all the species into seven different clusters.
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Acknowledgments
The authors are thankful to the Oil and Natural Gas Corporation Ltd (ONGC) and Oil India Ltd (OIL) for allowing us to collect soil samples from their oil fields in Upper Assam, India. The authors also thank Mr. Saurov Mahanta, Bioinformatics Division, DOEACC Centre Guwahati, Assam, India for helping with the software used in the study.
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Tanti, B., Ray, S.K. & Buragohain, A.K. Differentiation of petroleum hydrocarbon-degrading Pseudomonas spp. based on PCR-RFLP of the 16S-23S rDNA intergenic spacer region. Folia Microbiol 57, 47–52 (2012). https://doi.org/10.1007/s12223-011-0088-z
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DOI: https://doi.org/10.1007/s12223-011-0088-z