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Reliability of nucleotide sequence information of full-length flagellin A gene (flaA) and flaA short variable region (SVR) for molecular discrimination of Campylobacter lari organisms

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Abstract

We aimed to clarify if the thermophilic Campylobacter lari organisms including urease-negative (UN) C. lari and urease-positive thermophilic Campylobacter (UPTC) can be differentiated at the species and/or subspecies levels by employing the full-length flaA gene and flaA short variable region (SVR) nucleotide sequence information or not. Thermophilic Campylobacter isolates (n = 45) including UN C. lari (n = 17), UPTC (n = 18), and Campylobacter jejuni (n = 10) were well discriminated at the isolate level by the unweighted pair group method using arithmetic means analysis and neighbor joining procedures constructed based on the full-length flaA gene and flaA SVR nucleotide sequence information. Thus, these procedures may possibly be useful for epidemimological studies for C. lari and C. jejuni.

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Abbreviations

MLEE:

Multilocus enzyme electrophoresis

NARTC:

Nalidixic acid resistant thermophilic Campylobacter

PFGE:

Pulsed-field gel electrophoresis

SVR:

Short variable region

UN:

Urease-negative (isolates)

UPTC:

Urease-positive thermophilic Campylobacter

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Authors and Affiliations

  1. Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Chuo-ku, Sagamihara, 252–5201, Japan

    J. Hirayama, A. Tazumi, S. Nakanishi, E. Tasaki, K. Hayashi, H. Ueno, T. Kuribayashi & M. Matsuda

  2. School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, UK

    J. E. Moore

  3. Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast, BT9 7AD, UK

    J. E. Moore & B. C. Millar

Authors
  1. J. Hirayama
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  2. A. Tazumi
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  3. S. Nakanishi
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  4. E. Tasaki
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  5. K. Hayashi
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  6. J. E. Moore
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  7. H. Ueno
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  8. B. C. Millar
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  9. T. Kuribayashi
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  10. M. Matsuda
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Corresponding author

Correspondence to M. Matsuda.

Additional information

J. Hirayama and A. Tazumi contributed equally to this manuscript and hence should be considered joint first authors.

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Hirayama, J., Tazumi, A., Nakanishi, S. et al. Reliability of nucleotide sequence information of full-length flagellin A gene (flaA) and flaA short variable region (SVR) for molecular discrimination of Campylobacter lari organisms. Folia Microbiol 56, 103–109 (2011). https://doi.org/10.1007/s12223-011-0026-0

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  • DOI: https://doi.org/10.1007/s12223-011-0026-0

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