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Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia

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Abstract

A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.

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Abbreviations

Ap:

Ampicilin

Ara:

Arabinose

BHR:

Broad host range

C23O:

Catechol 2,3-dioxygenase (EC 1.13.11.2)

Km:

Kanamycin

LB:

Luria–Bertani

MCS:

Multiple cloning site(s)

R:

Resistance/resistant

Tet:

Tetracycline

xylE :

Gene encoding C230

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Acknowledgments

This research was supported by grant CMU-99-S-42 from the China Medical University and grant NSC 98-2320-B-039-011-MY3 from the National Science Council.

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Authors and Affiliations

  1. Graduate Institute of Microbiology and Public Health, National Chung-Hsing University, Taichung, 402, Taiwan

    Yi-Wei Huang & Tung-Ching Chung

  2. Department of Biotechnology, Asia University, Taichung, 413, Taiwan

    Rouh-Mei Hu

  3. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, 404, Taiwan

    Yu-Ting Chiang & Tsuey-Ching Yang

  4. School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, 110, Taiwan

    Tsao-Chuen Chung

  5. Department of Veterinary Medicine, National Chung-Hsing University, 250, Kuo Kuang Rd, Taichung, 402, Taiwan, Republic of China

    Tung-Ching Chung

  6. 91 Hsueh-Shih Rd, Taichung, 40402, Taiwan, Republic of China

    Tung-Ching Chung & Tsuey-Ching Yang

Authors
  1. Yi-Wei Huang
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  2. Rouh-Mei Hu
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  3. Yu-Ting Chiang
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  4. Tsao-Chuen Chung
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  5. Tung-Ching Chung
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  6. Tsuey-Ching Yang
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Corresponding authors

Correspondence to Tung-Ching Chung or Tsuey-Ching Yang.

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Huang, YW., Hu, RM., Chiang, YT. et al. Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia . Folia Microbiol 56, 18–22 (2011). https://doi.org/10.1007/s12223-011-0008-2

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  • DOI: https://doi.org/10.1007/s12223-011-0008-2

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