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J774 macrophage-like cell line cytokine and chemokine patterns are modulated by Francisella tularensis LVS strain infection

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Abstract

Mutual interactions were investigated between intracellular parasitic bacterium Francisella tularensis (F.t.; highly virulent bacterium responsible for tularemia, replicating within the host macrophages) and murine macrophage-like cell line J774. Recombinant murine lymphokine INF-γ and/or LPS derived from E. coli were determined to stimulate in vitro antimicrobial activity of macrophage-like J774 cell line against the live vaccine strain (LVS) of F.t. through their ability to produce proinflammatory cytokines and chemokines. F.t. infection up-regulated IL-12 p40 production and down-regulated TNF-α production by stimulated macrophages; on the other hand, F.t. infection did not affect the production of IL-8, IL-6, MCP-5, and RANTES by stimulated macrophages. This showed that F.t. infection modulates the cytokine synthesis by J774 macrophage cell line.

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Abbreviations

BSA:

fetal bovine serum (bovine serum albumin)

CFU:

colony-forming units

DMEM:

Dulbecco’s modified Eagle medium

ELISA:

enzyme-linked immunosorbent assay

F.t. :

Francisella tularensis

FasL:

Fas ligand (chemokines)

IL:

interleukin

IL-12 p40:

IL-12 p40 subunit

LPS:

lipopolysaccharide

LVS:

live vaccine strain

MCP-5:

monocyte chemotactic protein 5

MIP 1α/β:

macrophage inflammatory protein

NFκB:

nuclear factor-κB

NK:

natural killer (cells)

PAMP(s):

pathogen-associated molecular pattern(s)

PBS:

phosphate-buffered saline

PRR(s):

pattern recognition receptor(s)

RANTES:

regulated upon activation, normal T cell expressed, and presumably secreted

TLR:

Toll-like receptors

TNF-α:

tumor necrosis factor α

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Authors and Affiliations

  1. Department of Clinical Immunology and Allergology, University Hospital and School of Medicine in Hradec Kralove, Charles University in Prague, 500 05, Hradec Kralove, Czech Republic

    M. Holicka, J. Novosad, M. Kudlova, M. Loudova, C. Andrys & J. Krejsek

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  1. M. Holicka
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  2. J. Novosad
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  3. M. Kudlova
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  4. M. Loudova
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  5. C. Andrys
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  6. J. Krejsek
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Correspondence to J. Krejsek.

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Holicka, M., Novosad, J., Kudlova, M. et al. J774 macrophage-like cell line cytokine and chemokine patterns are modulated by Francisella tularensis LVS strain infection. Folia Microbiol 55, 191–200 (2010). https://doi.org/10.1007/s12223-010-0028-3

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  • DOI: https://doi.org/10.1007/s12223-010-0028-3

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