Abstract
The effect is described of selenium supplemented in an inorganic and organic form on the innate immune response of goats. Though the phagocytic activity (as a marker of the immune function) was found to be lower in organic-Se-treated group than in control (54.5 ± 4.32 vs. 60.2 ± 9.15 %), it did not generally exhibit any significant differences; similarly, no differences were found in the phagocytic index. The production of reactive oxygen species (ROS) was determined using the luminol-enhanced chemiluminescence (CL) (estimated as peak CL, integral CL and a peak time after addition of calcium ionophore A23187, opsonised zymosan (OZP) and phorbol-12-myristate-13-acetate as effectors. A significant ROS increase reflected in integral CL and a peak time was found in the inorganic-Se-treated group when OZP was used as activator; other parameters did not exhibit significant changes. The supplementation of Se in inorganic form can thus be seen to influence positively the innate immune system of kids.
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Abbreviations
- Ca-I:
-
calcium ionophore A23187
- CL:
-
chemiluminescence
- GPx:
-
glutathione peroxidase
- OZP:
-
opsonized zymosan
- PBS:
-
phosphate-buffered saline
- PMA:
-
phorbol-12-myristate-13-acetate
- RLU:
-
relative light unit(s)
- ROS:
-
reactive oxygen species
- WBC:
-
white blood cells
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Malá, S., Kovářů, F., Mišurová, Ľ. et al. Influence of selenium on innate immune response in kids. Folia Microbiol 54, 545–548 (2009). https://doi.org/10.1007/s12223-009-0079-5
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DOI: https://doi.org/10.1007/s12223-009-0079-5