Abstract
Bacillus vallismortis strain EXTN-1 is a proven biotic elicitor of systemic resistance in many crops against various pathogens. l-Alanine (Ala) was tested in cucumber as a chemical elicitor of induced systemic resistance (ISR) against Colletotrichum orbiculare. In the greenhouse, both Ala and EXTN-1 induced significant levels of disease suppression in cucumber against anthracnose. When cucumber plants were treated with EXTN-1 and Ala together, augmentative disease suppression was observed. Experiments with transgenic tobacco plants carrying pathogenesis-related genes fused with the β-glucuronidase (GUS) reported gene (PR-1a::GUS & PDF 1.2::GUS) showed an enhanced activation of both PR-1a and PDF 1.2 genes upon combined treatment with Ala and EXTN-1. RT-PCR analysis with transgenic (PR-1a or PDF 1.2 over expressing) Arabidopsis plant showed more enhanced expression of resistance genes PR-1a and PDF 1.2 upon combined treatment with Ala and EXTN-1 than either alone. An augmentative ISR effect, when the bacterial elicitor and chemical elicitor were combined together, was confirmed.
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Abbreviations
- Ala:
-
l-alanine
- BTH:
-
2,1,3-benzothiadiazole
- GUS:
-
β-glucuronidase
- ISR:
-
induced systemic resistance
- Jas:
-
jasmonic acid (2-(cis-2-pentenyl)-3-carboxymethylcyclopentanone)
- RT-PCR:
-
reverse transcriptase polymerase chain reaction
- Sal:
-
salicylic acid (2-hydroxybenzoic acid)
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Park, K.S., Paul, D., Kim, J.S. et al. l-Alanine augments rhizobacteria-induced systemic resistance in cucumber. Folia Microbiol 54, 322–326 (2009). https://doi.org/10.1007/s12223-009-0041-6
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DOI: https://doi.org/10.1007/s12223-009-0041-6