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Role of Yersinia pseudotuberculosis outer proteins (Yops) in murine humoral immune response

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Abstract

The infection of mice with the wild-type (WT) strain of Y. pseudotuberculosis did not induce polyclonal activation of B lymphocytes. Suppression in the production of certain isotypes of Ig was observed, provoked mainly by YopH, YopJ and YpkA. The WT strain induced a progressive increase in the serum-specific IgG, which peaked after 4 weeks after infection, IgM being produced only after 1 week. Autoantibodies against phosphorylcholine, myelin, thyroglobulin and cardiolipin could be detected in the serum of mice infected with the WT strain. The infection of mice provoked suppression in the production of immunoglobulins by splenic B cells and that YopH, YopJ and YpkA must be involved here.

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Abbreviations

Ab(s):

antibody(ies)

BCIP:

5-bromo-4-chloro-3-indolylphosphate

BSA:

bovine serum albumin

ELISA:

enzyme-linked immunosorbent assay

ELISPOT:

enzyme-linked spot assay

ERK:

extracellular receptor-activated kinase

HRP:

horseradish peroxidase

ICAM-1:

intercellular adhesion molecule-1

Ig:

immunoglobulin

IL-8:

interleukin 8

JNK:

c-Jun N-terminal kinase

LAT:

linker for activation of T cells

LD50 :

lethal dose 50 %

MAPK:

mitogen-activated protein kinases

NF-κB:

nuclear factor κB

NK:

natural killer cell

PBS:

phosphate-buffered saline

PBS/T PBS:

containing 500 ppm Tween 20

PBS/T/BSA PBS/T:

containing 1 % BSA

PVDF:

polyvinylidene fluoride

RANTES:

regulated by activation, normal T cell expressed and secreted

SLP-76:

SH2-domain-containing leukocyte protein of 76 kDa

TCR:

T cell antigen receptor

TNF-α:

tumor necrosis factor α

WT:

wild type

Yops:

Yersinia outer proteins

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Authors and Affiliations

  1. Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, SP, Brazil

    J. M. L. Maia, L. G. S. Monnazzi & B. M. M. Medeiros

  2. Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, UNESP, Rodovia Arara-quara-Jaú, Km 1, 14801-902, Araraquara, SP, Brasil

    B. M. M. Medeiros

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  1. J. M. L. Maia
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  2. L. G. S. Monnazzi
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Correspondence to B. M. M. Medeiros.

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Maia, J.M.L., Monnazzi, L.G.S. & Medeiros, B.M.M. Role of Yersinia pseudotuberculosis outer proteins (Yops) in murine humoral immune response. Folia Microbiol 54, 239–245 (2009). https://doi.org/10.1007/s12223-009-0038-1

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