Abstract
Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (±)-catechin (Cat) was tested for the storage of three commercial wine yeasts at −20 °C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A decline in survival throughout the storage period was observed. The media containing Asc maintained viability better than the other three. The BM caused a loss of viability of 7 orders for one strain and of 6 orders for the other two. All three strains exhibited a loss of viability of 4 orders when stored in BM+Asc. Two strains decreased viability by 5 orders while one strain by 4 orders, when stored in BM+Cat. Two strains decreased viability by 6 orders while one strain by 5 orders, when stored in BM+Asc+Cat. Regarding the physical state of the medium tested on 706 yeast strains, three cases were observed: completely liquid (56.5 %), liquid with only the upper part frozen (40.4 %) without involving the yeast biomass settled at the bottom, and completely frozen (3.12 %). It is practicable to prepare a BM that remains liquid at −20 °C enhancing yeast viability when Asc is added as cryoprotectant.
Similar content being viewed by others
Abbreviations
- Asc:
-
ascorbic acid
- BM:
-
basic medium
- Cat:
-
(±)-catechin
References
Caridi A.: Protective agents used to reverse the metabolic changes induced in wine yeasts by concomitant osmotic and thermal stress. Lett.Appl.Microbiol. 35, 98–101 (2002).
Caridi A.: Effect of protectants on the fermentation performance of wine yeasts subjected to osmotic stress. Food Technol.Biotech. 41, 145–148 (2003).
Caridi A.: Enological functions of parietal yeast mannoproteins. Antonie van Leeuwenhoek Internat.J. G 89, 417–422 (2006).
Castro H.P., Teixeira P.M., Kirby R.: Changes in the membrane of Lactobacillus bulgaricus during storage following freeze drying. Biotechnol.Lett. 18, 99–104 (1996).
Espinel-ingroff A., Montero D., Martin-mazuelos E.: Long-term preservation of fungal isolates in commercial prepared cryogenic microbank vials. J.Clin.Microbiol. 42, 1257–1259 (2004).
Gimeno-alcañiz J.V., Matallana E.: Performance of industrial strain of Saccharomyces cerevisiae during wine fermentation is affected by manipulation strategies based on sporulation. Syst.Appl.Microbiol. 24, 639–644 (2001).
Homolka L., Lisá L.: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials — an Alternative for Agar Slants. Folia Microbiol. 53, 534–536 (2008).
Homolka L., Lisá L., Kubátová A., Váňová M., Janderová B., Nerud F.: Cryopreservation of filamentous micromycetes and yeasts using perlite. Folia Microbiol. 52, 153–157 (2007).
Hubálek Z.: Protectants used in the cryopreservation of microorganisms. Cryobiology 46, 205–229 (2003).
Kagaya N., Hara Y., Saijo R., Kamiyoshi A., Tagawa Y.I., Kawase M., Yagi K.: Novel function of rare catechin, epigallocatechin-3-(3″-O-methyl)gallate, against cold injury in primary rat hepatocytes. J.Biosci.Bioeng. 96, 559–563 (2003).
Kawase M., Wang R., Shiomi T., Saijo R., Yagi K.: Antioxidative activity of (−)-epigallocatechin-3-(3″-O-methyl)gallate isolated from fresh tea leaf and preliminary results on its biological activity. Biosci.Biotech.Biochem. 64, 2218–2220 (2000).
Kupiec M., Byers B., Esposito R., Mitchell A.: Meiosis and sporulation in Saccharomyces cerevisiae, pp. 889–1036 in J. Pringle, J. Broach, E. Jones (Eds): Cell Cycle and Cell Biology. Cold Spring Harbor Laboratory Press, New York 1997.
Rakauskaite R., Citavicius D.: The genetic control of cell growth and development in yeast Saccharomyces cerevisiae: disturbed sporulation in diploids with a decreased activity of the Ras/cAMP signal transduction pathway. Russ.J.Genet. 39, 610–617 (2003).
Russell I., Stewart G.G.: Liquid nitrogen storage of yeast cultures compared to more traditional storage methods. Am.Soc.Brewing Chemists J. 39, 19–24 (1981).
Schmidt J.L., Diez M., Lenoir J.: Conservation par congélation de levures et de moisissures d’origine fromagère. Sci.Aliment. 11, 653–672 (1991).
Sherman F., Fink G.R., Hicks J.B.: The Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, New York 1986.
Stoycheva T., Venkov P., Tsvetkov Ts.: Mutagenic effect of freezing on mitochondrial DNA of Saccharomyces cerevisiae. Cryobiology 54, 243–250 (2007).
Tatchell K., Robinson L.C., Breitenbach M.: RAS2 of Saccharomyces cerevisiae is required for gluconeogenesis growth and proper response to nutrient limitation. Proc.Acad.Nat.Sci.Phil. 82, 3785–3789 (1985).
Zárate G., Nader-Macias M.E.: Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatine capsules. Process Biochem. 41, 1779–1785 (2006).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sidari, R., Caridi, A. Viability of commercial wine yeasts during freezer storage in glycerol-based media. Folia Microbiol 54, 230–232 (2009). https://doi.org/10.1007/s12223-009-0036-3
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12223-009-0036-3