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Effect of growth temperature and pH on the aminopeptidase activity of Pseudomonas putida, P. fluorescens and Flavobacterium odoratum; the 4-nitroaniline test is reliable

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Abstract

No significant difference (p > 0.05) was observed in the specific aminopeptidase activity (SAA) developed by Pseudomonas fluorescens, P. putida and Flavobacterium odoratum either growing at pH 5.0–6.5 or at 7 and 12 °C. Nevertheless, a significant difference was found when comparing the SAA of these organisms. The SAA of F. odoratum was lower than those of pseudomonads. The 4-nitroaniline test is reliable to estimate the G load of fresh food products.

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Abbreviations

SAA(s):

specific aminopeptidase activity(ies)

TSB:

tryptone soy broth

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Correspondence to G. Garcia de Fernando.

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Lopez-Tomas, L.A., Ordoñez, J.A., Mediavilla, C. et al. Effect of growth temperature and pH on the aminopeptidase activity of Pseudomonas putida, P. fluorescens and Flavobacterium odoratum; the 4-nitroaniline test is reliable. Folia Microbiol 53, 423–426 (2008). https://doi.org/10.1007/s12223-008-0064-4

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